摘要
将来自Brucellaabortus的葡萄糖激酶基因glk和大肠杆菌乳糖操纵子基因lacZYA装置于蓝藻glnA启动子下游 ,以广宿主质粒导入 3种蓝藻。基因的表达通过 β 半乳糖苷酶和葡糖激酶活性测定得到确证。基因工程藻株均获得了利用乳糖异养生长的能力。集胞藻 6 80 3转基因株可在光照加DCMU的条件下利用乳糖生长 ,而鱼腥藻转基因株依赖乳糖可在完全黑暗条件下生长 。
The glucose kinase gene glk from Brucella abortus and lacZYA genes from E. coli were positioned downstream of a cyanobacterial glnA promoter, and introduced into three cyanobacterial species in a broad-host-range plasmid. Expression of the exogenous genes was confirmed by assay of β-galactosidase and glucokinase activity. The genetically-engineered cyanobacterial strains acquired capability of heterotrophic growth on lactose. Synechocystis sp. PCC 6803 (pRL2224) can grow on lactose in the light with DCMU, while Anabaena strains harboring pRL2224 are capable of heterotrophic growth in complete darkness and produce heterocysts upon induction in darkness or in the light with DCMU.
出处
《水生生物学报》
CAS
CSCD
北大核心
2002年第4期363-369,共7页
Acta Hydrobiologica Sinica
基金
中国科学院"百人计划"
生物领域创新青年科学家小组资助项目
