摘要
目的 :探讨硒对高氟所致雄性大鼠生殖损害的拮抗作用。方法 :1 0 0只雄性Wistar大鼠随机分成对照组、氟 (NaF 1 5 0mg/L)组、氟加低硒 (0 .5mg/L亚硒酸钠 )组、氟 (NaF 1 5 0mg/L)加中硒 (2 .0mg/L亚硒酸钠 )组、氟(NaF 1 5 0mg/L)加高硒 (4 .0mg/L亚硒酸钠 )组 5组 ,通过饮水加氟、加硒的方法染毒 1 0周。观察氟、硒对大鼠体重、睾丸和附睾质量及其脏器系数 ,精子计数、活动率及畸形率 ,睾丸、附睾生化标志酶 ,血清性激素 ,睾丸组织病理切片以及初级精母细胞染色体畸形率的影响。结果 :①右侧睾丸质量对照组为 (1 .80± 0 .2 2 )g ,氟加高硒组为 (1 .6 1±0 .1 1 )g ,2者相比差异有统计学意义 (P <0 .0 5 )。②精子计数 :氟组 (9.2 3× 1 0 7ml-1 )、氟加中硒组 (37.6 7× 1 0 7ml-1 )与对照组 (2 8.6 0× 1 0 7ml-1 )比较 ,差异有统计学意义 (P <0 .0 0 1 ) ;精子畸形率 :氟组 (36 .33% )氟加中硒组 (1 2 .70 % )与对照组 (1 4 .83% )比较 ,差异有统计学意义 (P <0 .0 0 1 ) ;精子活动率 :氟组 (6 2 .0 7%± 6 .5 0 % )氟加中硒组 (6 8.2 1 %± 4 .99% )与对照组 (73.6 7%± 6 .5 1 % )比较 ,差异有统计学意义 (P <0 .0 0 1 )。③LDH :氟组 ((5 79.4 7± 97.1 6 )u/g)、氟加中硒组 ((72 6 .1 3± 1
Aim: To study the antagonism of selenium on male reproductive toxicity induced by excessive fluorine. Methods:A total of 100 male Wistar rats were randomly allocated into five groups: control group, fluorine group, fluorine plus low-dose selenium, fluorine plus mid-dose selenium and fluorine with high-dose selenium, then the animals were fed deionized water supplemented with NaF or/and Na 2SeO 3 for ten weeks. The body weights, right testical, right epididymitis and its organic coefficient were measured and calculated; the count of sperm and the rates of sperm mobility and aberration were analyzed; the levels of lactate dehydrogenase(LDH),Na +K +-ATPase, Mg 2+ -ATPase, Ca 2+ -ATPase and serum testosterone(T), luteinizing hormone(LH) were examined; meanwhile the pathological alterations of testes and the chromosome aberration of primary spermatocytes were observed. Result: Weight of right testical was significantly reduced ( P <0.05) in group of fluorine plus high-dose selenium (1.61±0.11)g compared to control (1.80±0.22)g. Sperm count in fluorine group (9.23×10 7 ml -1 ) and fluorine plus mid-dose selenium group (37.67×10 7 ml -1 ) compared to control (28.60×10 7 ml -1 ) had significant differences; significant differences ( P <0.001) were also observed in fluorine group (36.33%) and fluorine plus mid-dose selenium group (12.70%) compared to control rats (14.83%) in the aberration rate of sperm; meanwhile significant differences ( P <0.001) were observed between fluorine group (62.07%±6.50%) and fluorine plus mid-dose selenium group (68.21%±4.99%) compared to control group (73.67%±6.51%) in the rate of sperm mobility. The level of LDH was significantly ( P <0.001) decreased in fluorine (579.47±97.16)u/g and fluorine plus mid-dose selenium (726.13±115.55)u/g compared to control (736.80±86.03)u/g; similar significant ( P < 0.001) changes were found in the level of Na +K +-ATPase in the three groups ((1.04±0.18) μmol·mg -1 ·h -1 , (1.28±0.12) μmol·mg -1 ·h -1 , (1.37±0.21) μmol·mg -1 ·h -1 ); while the level of Mg 2+ -ATPase in the four groups administered fluorine or/and selenium had significant ( P <0.001) differences from that of control. Significant differences ( P <0.05) were observed in fluorine plus mid-dose selenium group (422.3 ng·L -1 ) and fluorine plus high-dose selenium group (339.0 ng·L -1 ) compared to control (260.7 ng·L -1 ) in the T level in serum; and the LH level was significantly ( P <0.05) changed in the fluorine plus low-, mid-, and high-dose selenium groups compared to control respectively. The microstructure of testis was loosened in fluorine group compared to control rats, while the pathological alterations of testis induced by fluoride were counteracted in the fluorine plus low-, mid-, and high-dose selenium groups compared to fluorine group. No significant difference ( P >0.05) was observed after fluorine and/or selenium treatments in the rate of chromosome aberration of primary spermatocytes compared to control group.Conclusions: Fluoride can result in reproductive toxicity in male rats, and appropriate selenium takin can antagonize the toxicity of fluoride.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2003年第6期896-900,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省重大科技攻关资助项目 ( 1998-0 6)
关键词
氟
硒
拮抗作用
雄性生殖毒性
fluorine
selenium
antagonism
male reproductive toxicity
