摘要
目的 观察全反式维甲酸 (RA)和表皮生长因子 (EGF)对大鼠胚胎神经干细胞增殖和分化的影响。方法从大鼠胚胎脑中分离神经干细胞 ,经RA和EGF处理后 ,用台盼蓝确定细胞数量 ,BrdU标记分析细胞生长能力 ,采用免疫细胞化学法鉴定神经干细胞和分化的神经细胞。结果 2 0ng/mlEGF和 1μmol/LRA处理的培养细胞均显示增殖效应 ,但EGF处理组增殖速度明显高于RA组 ,悬浮细胞中有大量nestin和BrdU阳性细胞。用EGF和EGF/RA诱导的神经元分化率分别为 17%和 3 1% ,而RA处理的神经元分化率显著升高至 89%。由EGF、EGF/RA和RA诱导的星形胶质细胞分化率分别为 83 %、 69%和 11%。结论 EGF主要促进神经干细胞增殖并主要诱导星形胶质细胞的生成 ,RA主要诱导神经干细胞向神经元分化 。
Objective To investigate the effects of all-trans retinoic acid (RA) and epidermal growth factor (EGF) on the proliferation and differentiation of neural stem cells from rat embryonic brains. Method Neural stem cells were isolated from the brains of rat embryos and then treated with RA or EGF. Trypan blue dyeing, BrdU blinding and immunocytochemistry were carried out in this study. Result The effects of proliferation were found in the culture cells treated with 20ng/ml EGF and 1μmol/L RA, but the proliferative activity after EGF treatment was markedly higher than after present of RA, and nestin- and BrdU-positive cells were obtained in the suspension. The frequency of neuronal differentiation of retinal cells inducad by EGF and EGF/RA amounted to about 17% and 31% respectively, whereas the differentiation rate of neuronal cells induced by RA was significantly increased to 89%. The rate of astrocytes in the differentiated cells induced by EGF, EGF/RA and RA was 83%, 69% and 11% respectively. Conclusion These findings show that EGFmainly promotes the proliferation of neural stem cells and the differentiation into astrocytes and that RA mainly induces neural stem cells to differentiate into neurons in vitro. There are no cooperative effects between EGF and RA in inducing neural stem cells to proliferate and differentiate.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2003年第1期81-85,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
河南省医学科技创新人才工程资助(No 2 0 0 2 10 7)
