摘要
目的 探讨碱性成纤维细胞生长因子 (bFGF)、表皮细胞生长因子 (EGF)、转化生长因子 β的三种异构体 (TGF β1,β2 和β3 )及它们的受体在不同发育阶段皮肤中的表达特征。方法 用免疫组化方法和病理学技术确定这些细胞因子及其受体在 30例不同胎龄(13～ 31周 )的胎儿皮肤和 5例成人皮肤中的定位和表达量的变化规律。结果 bFGF、EGF、TGF β异构体主要分布于表皮细胞、内皮细胞、毛囊上皮细胞和部分成纤维细胞的胞质和胞外基质中 ,而其受体则主要分布于这些细胞的细胞膜和细胞质内。随着胎儿不断生长发育 ,这些蛋白表达的阳性率逐渐升高 ,在晚期妊娠胎儿(胎龄 2 9～ 31周 )和成人皮肤中 ,蛋白表达进一步升高 ,阳性率明显高于早期妊娠胎儿皮肤。结论 bFGF、EGF、TGF β的异构体及其受体可能对皮肤的发生、结构和功能的维持、损伤后修复具有重要作用 ;在妊娠早期胎儿皮肤中 ,这些蛋白低表达可能与胎儿皮肤创面无瘢痕愈合密切相关。
Objective To observe the localization and expression characteristics of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), three transforming growth factor β (TGF β) isoforms, and their receptors in fetal and adult skin in order to explore their potential biological significance. Methods Protein expression and distribution of bFGF, EGF, three TGF β isoforms, and their receptors were observed with immunohistochemical method in 35 skin specimens, among which 30 specimens belonged to fetuses in different developmental stages and 5 were from adults. Results Positive immunohistochemical signals of bFGF, EGF, TGF β isoforms and their receptors could all be found in fetal and adult skins. These factors were mainly distributed in the cytoplasm and extracellular matrix of epidermal cells, endothelial cells, hair follicle epithelial cells and some fibroblasts. Receptors of these factors were mostly located in cellular membrane and cytoplasm of the cells mentioned above. Along with the increment of gestational age, the positive cellular rates of bFGF, EGF, three TGF β isoforms and their receptors in fetal skin were increased progressively. In skin specimens obtained from fetuses of late trimester pregnancy (29～31 week gestation) and adult, the positive rates of these proteins were significantly raised in comparison with skin from fetuses of early trimester pregnancy (13～17 week gestation)( P <0.05). Conclusion It is suggested that endogenous bFGF, EGF, three TGF β isoforms and their receptors might be involved in the development of skin at embryonic stage, maintenance of cutaneous structure and function, and also in wound healing at adult stage. The relative lack of these factors and their receptors in early gestational fetus may be one of the reasons that wounds in fetal skin heal by regeneration rather than by scarring.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第11期1001-1004,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家重大基础研究规划 (编号G1 9990 542 0 4 )
国家自然科学基金重点项目 (编号 30 2 30 370 )
国家自然科学基金 (编号 39870 731 )资助课题
