摘要
目的 :采用一种简便、快速的检测精子线粒体琥珀酸脱氢酶 (SDH)的改良方法检测精子SDH ,并评价其与精子活动率及存活率的关系。 方法 :4 6例年龄为 2 5～ 36岁 (平均 31岁 )生育与不育男性精液标本 ,采用计算机辅助精液分析系统检测精子活动率 ,应用改良SDH检测法检测精子SDH ,通过死、活精子荧光分子探针染色检测精子存活率 ,分析精子活动率、存活率及精子SDH阳性率之间的关系。 结果 :4 6例生育与不育男性精子活动率为( 6 7.33± 7.37) % ,存活率为 ( 79.78± 7.6 5 ) % ,精子SDH阳性率为 ( 74 .74± 8.2 9) % ;精子活动率、存活率及精子SDH阳性率 3者之间均存在显著相关性 (P均 <0 .0 1)。 结论 :精子线粒体SDH检测对评价精子线粒体功能具有重要意义 。
Objective: To detect succinate dehydrogenase (SDH) of sperm mitochondria by an improved method, and to evaluate the relationship between SDH and the motility and viability of sperm. Methods: In 46 fertile and infertile men (aged from 25 to 36), the motility, viability and mitochondrial SDH of sperm were detected by computer assisted semen analysis system, dead/live sperm molecular fluorescent probe and an improved cytochemical method. Then, the correlation between the motility and viability of sperm and the positive percentage of sperm mitochondrial SDH were analyzed. Results: In the 46 fertile and infertile men, the motility and viability of sperm were ( 67.33 ± 7.37 )% and ( 79.78 ± 7.65 )%, and the positive percentage of sperm mitochondrial SDH was ( 74.74 ± 8.29 )%. The motility and viability of sperm and positive percentage of sperm mitochondrial SDH had significant correlation ( r = 0.901 , P < 0.01 ; r = 0.876 , P < 0.01 ; r = 0.917 , P < 0.01 ). Conclusions: Detection of sperm mitochondrial SDH has significance in evaluation of sperm mitochondrial function and may serve as an assisted marker of sperm viability.
出处
《中华男科学杂志》
CAS
CSCD
2003年第8期601-603,共3页
National Journal of Andrology
关键词
琥珀酸脱氢酶
线粒体
活动率
存活率
精子
Succinate dehydrogenase
Mitochondria
Motility
Viability
Sperm
