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DEFENSE MECHANISMS OF URINARY BLADDER:STUDIES ON ANTIMICROBIAL POLYPEPTIDES FROM BLADDER MUCOSA 被引量:1

DEFENSE MECHANISMS OF URINARY BLADDER: STUDIES ON ANTIMICROBIAL POLYPEPTIDES FROM BLADDER MUCOSA △
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摘要 The acid soluble extract of the bladder mucosal surface was obtained by washing out the bladder with dilute acetic acid in the presence of protease inhibitors. The wash out materials from rats, rabbits, pigs, and humans manifested strong bactericidal activity against E.coli in vitro. The ultrafiltrate of the human material, which contained two major peptides with apparent molecular masses of 6 7 kD and 8 5 kD, respectively, showed potent bactericidal activity against E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus sanguis.Three antibacterial polypeptides (PiBPs) were purified from the porcine material. The molecular masses of PiBP 5, PiBP 11 and PiBP 25 were 5773.3 Da, 11127.8 Da and 25073 Da, respectively. PiBP 5 was unusually rich in glycine, serine and threonine residues(20 0, 16 3 and 10 4 mo1%, respectively), and N terminal amino acid sequencing revealed that PiBP 5 was homologous (83 3% identity in an 18 residue overlay) to the “tail” of human cytokeratin 7. Although the amino acid compositions of PiBP 11 and PiBP 25 were established, both had blocked N termini and primary sequence data were not obtained. These results provided evidence indicating that the presence of peptides in the bladder mucosa could enable it to kill adherent bacteria.
作者 吴琦 王伯瑶
出处 《Chinese Medical Sciences Journal》 CAS CSCD 1999年第1期17-22,共6页 中国医学科学杂志(英文版)
关键词 urinary bladder defense mechanisms antimicrobial polypeptides 多肽类 抗菌素 膀胱粘膜 动物试验 缩氨酸
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