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鹅细小病毒不同毒株VP3基因的序列分析比较 被引量:12

Comparing and analyzing of the VP3 gene sequence of different goose parvovirus strains
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摘要 分别将GPV 4个分离株通过PCR技术 ,从病毒基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段 ,并与PMD18_T质粒载体连接 ,转化到感受态大肠秆菌TG1中 ,提取重组质粒经PCR鉴定和酶切鉴定后 ,对插入片段进行序列测定及分析。结果表明 :VP3基因全长 16 0 5bp ,编码 5 34个氨基酸。不同毒株主要结构蛋白VP3基因同源性较高(95 .6 4 %~ 99.81% )。但强弱毒株间也存在一定差异。 VP3 gene of different Goose parvovirus strains were amplified from the DNA by Polymerase Chain Reaction (PCR), the Vp3 genes were cloned into PMD 18-T vector respectively. The recombinant plasmid were identified with restriction endonuclease analysis and PCR, and then sequenced.The result of sequence analysis showed that VP3 gene had 1 605 bp and included a complete open reading frame which encoding a protein of 534 amino acids,and all the four strains of GPV shared highly common identity(95.64 %~99.81 %),but there is also some difference between highly lethal strains and mild strains.
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2003年第6期430-433,共4页 Chinese Journal of Preventive Veterinary Medicine
关键词 鹅细小病毒 VP3基因 克隆 序列分析 goose parvovirus, VP3 gene, cloning, sequencing.
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  • 1陈常亮,段谊章.小鹅瘟免疫水平的研究[J]中国兽医杂志,1988(07).
  • 2周阳生,田慧芳,过建寅,方定一.小鹅瘟疫苗对初生雏鹅的安全性及免疫力试验[J]中国兽医杂志,1984(09).

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