摘要
目的 :建立HPLC快速测定血清茶碱浓度的方法。方法 :色谱柱 μ BondapakC18(4.6mm× 2 5 0mm ,10 μm) ,流动相甲醇 水 (2 5∶75 ) ,流速 1.2mL·min-1,检测波长 2 6 5nm ,以乙酰苯胺为内标 ,血清用乙腈沉淀蛋白后进样分析。结果 :茶碱在2 .5～ 4 0mg·L-1浓度范围之间线性良好 ,r =0 .9994。平均回收率为 99.5 %。对 34例患者作血清茶碱浓度监测 ,临床效果满意。结论 :本方法取样量少 ,操作简便 ,可作为茶碱血药浓度监测的常规方法。
OBJECTIVE To determine theophlline rapidly in serum by HPLC.METHODS HPLC was slected with acetanilide as internal standard to determine concentration of theophylline in serum. The separation was performed on μ-BondapakC18( 4.6 mm×250 mm,10 μm)column with methanol-water(25∶75) as mobile phase.The flow rate was 1.2 mL·min -1 .The wavelength for detection was 265 nm .Ethyl acetate was used to precipitate the serum protein.RESULTS The calibration curve had good linearity between 2.5 ～40 mg·L -1 , r = 0.9994 .The average recovery was 99.5% .The clinical application was good.CONCLUSIONS This method is accuate ,rapid and simple.It can be used as a roution method by TDM.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2003年第12期738-739,共2页
Chinese Journal of Hospital Pharmacy
