摘要
利用摇瓶在温度、pH范围、诱导剂量及诱导时间等不同因素变化时,进行了重组毒素LHRH-PE40工程菌发酵条件的初步探索.结果表明,工程菌优化的发酵条件为:pH范围7.3～7.5,诱导温度为34 ℃.诱导时菌密度A600=0.8,诱导时间为4～4.5 h,诱导剂(IPTG)浓度为0.5 mmol/L.
The basic fermentation conditions of the recombinant toxin(LHRH-PE40) in E.coli were studied in shake flask scale. Some optimized fermentation conditions were obtained through the experiments: pH was adjusted between (7.3)～(7.5). The cell concentration was _A_(600)=(0.75), induction temperature was 34?℃, induction time was 4～(4.5), and inducer concentration was (0.5)?mmol/L.
出处
《北华大学学报（自然科学版）》
CAS
2003年第6期484-486,共3页
Journal of Beihua University（Natural Science）
