摘要
目的 :研究肛门直肠畸形患儿直肠末端的基因表达谱 ,初步筛查肛门直肠畸形的差异表达基因。方法 :应用基因表达谱芯片技术对 3例先天性肛门直肠畸形患儿的直肠末端组织和 3例正常直肠末端组织的基因表达进行检测。结果 :在 186个发育相关基因中 ,共发现差异表达基因 5 4个 ,表达上调的 5 2个 ,表达下调的 2个。结论 :先天性肛门直肠畸形的发生涉及多基因改变。基因表达谱芯片技术可同时定量研究大量基因表达水平 。
Objective: Our purpose was to study the profile of gene expression in distal part of rectum in infants with anorectal malformation.Methods:The cDNA microarray was used to detect the gene expression of 3 infants with anorectal malformation and normal rectal tissues of 3 infants without the malformation.Results:Among 186 developmental genes, there were 54 differences, of which 52 were up regulated and 2 down regulated in infants with anorectal malformation. Conclusion:Anorectal malformation has a multifactorial and polygenic background. The cDNA microarray techniques are rapid and effective in screening differential gene expression.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2003年第6期493-495,共3页
Journal of China Medical University
关键词
人类肛门直肠畸形
基因芯片
基因表达谱
human anorectal malformation
cDNA microarray
profile of gene expression
