摘要
ＲＥＤＵＣＥＤＴＵＭＯＲＩＧＥＮＩＣＩＴＹＯＦＭＥＴＡＳＴＡＴＩＣＨＵＭＡＮＬＵＮＧＣＡＮＣＥＲＣＥＬＬＳＵＢＬＩＮＥ（ＰＧＣＬ３）ＴＲＡＮＳＦＥＣＴＥＤＷＩＴＨｈＲＡＲβＧＥＮＥＺｈｕＷｅｉｙｏｎｇ朱伟勇；ＷｕＢｉｎｇｑｕａｎ吴秉铨；ＺｈｅｎｇＪｉ...
The recombinant PSG5-RARβ plasmid and the G418-resistant PSV2neo Plasmid ( 10 : 1) were cotransfected into PGCL3 cells by copreciprtation with calcium phosphate. The transfectants CR3 and CR4, which expressed the RARβ gene. were identified by Northern blot hybridization. The results showed that the in vitro growth and invasion of CR3 and CR4 were dramatically reduced compared to the control-transfected cell (CSV1). Furthermore, the colony-forming abilities in soft agar and the tumorigenicrty in nude mice of CR3 and CR4 were abrogated. Our results suggests that RARβ functions not only as a receptor mediating the RA action, but also as a suppressor in lung tumorigenesis.
