摘要
ＨＬＡ－ＤＲＢ１ＡＬＬＥＬＥＳＧＥＮＯＴＹＰＩＮＧＩＮＰＡＴＩＥＮＴＳＷＩＴＨＲＨＥＵＭＡＴＯＩＤＡＲＴＨＲＩＴＩＳＩＮＣＨＩＮＥＳＥＺｈａｏＹａｎ（赵岩）；ＤｏｎｇＹｉ（董怡）；ＺｈｕＸｉｌｉｎ（朱席林）ａｎｄＱｉｕＣｈａｎｇｃｈｕｎ（邱长春）（Ｎ...
Objective. To explore the role of HLA- DRBI genes in the development of rheumatoid arthritis(RA) and the orrelations between HLA-DR alleles and clinical manifestations of patients with RA.Methods. 86 patients with rheumatoid arthritis and 106 race matched controls were studied in whom HLA-DR typing was perfomed by the method of DNA amplification with sequence-specific primers(PCR-SSP). The subtypes of HLA- DR4 were determined by the method of hybridization of PCR products with sequence- specific oligonucletides(PCR-SSO) . The absence or presence of HLA-DR4 and its subtypes was correlated with the clinical and serological characteristics of the patients.Results. Compared with controls, an increased gene frequency of HLA-DR4 (48. 8% vs 17. 9 % , P<0. 001) and a decreased frequency of HLADR7 ( 16. 3% vs 27. 4 % , P = 0. 06 ) were found. The DRB1.0405 account for 61. 9 % of DR4+RA patients and 21. 1% of DR4+ controls(P<0. 01 ). There was no difference between the DR4+ and DR4- patients with respect to age ,sex ,duration of disease ,rheumatoid factor (RF) ,extra-articular manifestations including secondary Sjogren' s syndrome. According to the wrist X- ray stage,the patients of DR4+ were more severe than that of DR4- (P<0. 05).Conclusicon. HLA-DR4 and DR4 subtype of DRB1 0405 are related to the development of RA in Chinese. HLA-DR4 can be a useful prognostic marker in the patients with RA.
