摘要
采用滤纸片(直径9.5mm)取定量血样,风干,通过浸泡、萃取,进行雌二醇(17β—Estradiol)放射免疫测定(RIA),经与小塑料管微量法对比,二者标准曲线基本平行.微量法Y=4.27-2.24x,纸片法Y=4.35-2.11x,平均回收率为91.3%,批内CV=7.6%～9.8%(n=10),批间CV=15.1%～19.3%(n=4).纸片法与微量法相比,具有同样准确性、特异性,还具有完整性和可行性,其灵敏度可达10^(-12)～10^(-15)g.干血点纸片法采血量少,方法简便,样品易于保存和运输,可用于畜禽及牛奶中激素含量测定.
A sampling method for determinating E2 -17β in blood by radioimmunoassay was designed. A known volume of blood to be tested is added onto the filter paper disk (9. 5 mm in diameter) and allow it to dry in air at room temperature. When assaying, the sample can be extracted from the dry blood spotted disk. As compared with the microanalysis method, the standard curves are basically parallel. The microanalysis method, Y=4. 27-2. 24x; paper disk method, Y=4. 35 - 2.11. The average recovery rate is 91. 3%. Within batch, CV=7. 6%-9. 8% (n=10); between batches, CV=15. 1%-19. 3% (n=4). Its accuracy, specificity, integrity and feasibility are identical to those of microanalysis method. The sample disks may be stored and transported. It is suitable for determination of hormones in animals, fowls and milk.
