摘要
【目的】挖掘偃松种子萌发过程关键基因,为揭示偃松种子难于萌发的生理学和遗传学机制提供理论依据,并为进一步进行偃松资源开发利用提供技术保障。【方法】利用偃松种子萌发RNA-Seq数据,通过加权基因共表达网络分析的方法构建网络。【结果】进一步将该网络划分为4个模块,并寻找到2个特异性模块,分别为brown模块和turquoise模块。对两个模块进行GO富集分析和KEGG通路分析,发现二者均在代谢过程和细胞过程等生物学过程中发挥主要作用,其主要的细胞组分为细胞、细胞器和膜等,并均在催化反应、连接和运输中起显著作用;相比于turquoise模块,brown模块中基因信息处理过程所占比重有所提升,而相比于brown模块,turquoise模块在代谢过程中所占比重较大。利用cytoscape软件绘图,筛选特异性模块中的核心基因,并利用在线blast对其功能进行预测,其中brown模块的核心基因可能参与了ABA信号转导和固氮等过程;turquoise模块的核心基因可能参与了苯丙氨酸代谢途径、萜烯合成、果胶酶降解以及植物抗逆反应等。【结论】找到36个潜在的与偃松种子萌发相关的核心基因,其中brown模块基因为15个,turquoise模块基因为21个,但基因的具体功能有待于进一步的生物学验证。
【Objective】In order to provide a theoretical basis for revealing the physiological and genetic mechanism of the diffi cult germination of the seeds of Pinus pumila,and provide technical support for development and utilization of the resources of P. pumila,we detected the key genes of seed germination of P. pumila.【Method】A network was constructed by weighted gene co-expression network analyses(WGCNA)using RNA-Seq data of seed germination of P. pumila.【Result】The network was divided into 4 modules and two specific modules were found,which are the brown module and the turquoise module,respectively. Through the analysis of the GO concentration and the KEGG pathway of the two modules,it was found that both of the two modules played a major role in the biological processes such as the metabolic process and the cellular process. The main cellular components were the cell,the organelles and the membrane,etc.,and the main functions were catalytic activity,binding and transporter activity. Compared with the turquoise module,the proportion of genetic information processing in the brown module increased,while the turquoise module accounted for a large proportion in the metabolic compared to the brown module.The hub genes of specific modules were obtained by cytoscape,and calculate the function by online blast,which the hub genes of the brown module may be involved in the process of ABA signal transduction and nitrogen fi xation;the hub genes of the turquoise module may be involved in phenylalanine metabolism pathway,terpene synthesis,pectinase degradation and stress resistance of plant.【Conclusion】36 hub genes related to seed germination of P. pumila were identified,of which 15 were brown module bases and 21 were turquoise module bases,but the specific functions of the genes need to be further verified by biological means.
作者
王思瑶
丛日征
闫晓娜
于宏影
崔嵘
WANG Si-yao;CONG Ri-zheng;YAN Xiao-na;YU Hong-ying;CUI Rong(Harbin Research Institute of Forestry Machinery,National Forestry and Grassland Administration,Harbin 150086;Research Center of Cold Temperate Forestry,CAF,Harbin 150086)
出处
《温带林业研究》
2019年第1期39-46,共8页
Journal of Temperate Forestry Research
基金
中央级公益性科研院所基本科研业务费专项资金(CAFYBB2016ZD009
CAFYBB2017ZX001-6)
