摘要
目的探索北方某燃煤地区室外大气细颗粒物(PM_(2.5))水溶性离子对模式生物秀丽隐杆线虫的急性致死效应,并探究其对线虫生长发育、神经行为和衰老进程的影响。方法以空气质量等级为优、轻度、中度和重度污染时采集的燃煤地区PM_(2.5)水溶液样本(浓度当量分别为17.5、101.4、130.7和188.9μg/ml,对应于优、轻、中和重组)对同步化的L1和L4期线虫分别进行染毒,测定其存活率、体长、体宽、咽泵振动频率、肠道自发荧光和热耐受力变化。结果 L1期线虫染毒72 h后,优组、轻组和中组的体长均有改变(P<0.05),重组的体宽差异有统计学意义(P<0.01),轻、中和重组的自发荧光强度均比对照组明显增强(P<0.01);L4期线虫染毒24 h,中度和重度污染组体长体宽、咽泵频率的改变与对照组比较,差异有统计学意义(P<0.01),重度污染组肠道自发荧光比对照组明显增强(P<0.01),继续染毒至72 h时优组和重组肠道荧光明显增强(P<0.01),染毒至第5天时各染毒组在热应激条件下平均寿命明显缩短(P<0.05),染毒至10天时轻度和重度污染组热耐受力比对照组明显减弱,差异有统计学意义(P<0.01)。结论燃煤区来源的PM_(2.5)可对线虫生长发育、神经运动等产生毒性效应,并加速线虫的衰老进程,提示有必要进一步开展其毒性分子机制研究。
Objective To study the toxic effects of water-soluble PM2.5 exposure on survival,growth and development,neuron function and aging on C.elegans,and evaluate the toxicity of the water-soluble components of outdoor PM2.5 samples from a coal-fired region in northern China.Methods Nematodes at L1 stage or L4 stage were exposed to PM2.5(group-E,group-L,group-M,group-H,with concentration of 17.5,101.4,130.7 and 188.9μg/ml respectively),the survival rate,body length and width,pharyngeal pump vibration frequency were recorded,and the level of intestinal autofluorescence as well as heat tolerance were measured.Result PM2.5 didn’t induce acute lethal toxicity to C.elegans.For L1 stage nematodes,the body length of group-E,group-L and group-M were statistically different with that from control group(P<0.05),and the body width of group-H was statistically significant(P<0.01).The intestinal fluorescence of group-L,group-M and group-H were significantly higher than that of the control group(P<0.01).For nematodes at L4 stage,the body size and pharyngeal pump frequency of group-M and group-H were statistically different from the control group;At 24 h the intestinal autofluorescence in group-H increased significantly(P<0.01),and the fluorescence of group-E and group-H were significantly increased when the exposure was continued for 72 hours(P<0.01).The average lifespan of the exposed groups was significantly shortened under heat stress on day 5(P<0.05),and the heat tolerance of group-L and group-H were significantly weaker than that of the control group on day 10(P<0.01).Conclusion PM2.5 from coal combustion could induce toxic effects on nematode growth and nerve function,furthermore,accelerate the aging process of nematodes,which suggesting that further study on its molecular mechanism is necessary.
作者
张文静
张楠
李国君
敬海明
李子南
宁钧宇
高珊
娄云
王心宇
陶晶
张永
ZHANG Wen-jing;ZHANG Nan;LI Guo-jun;JING Hai-ming;LI Zi-nan;NING Jun-yu;GAO Shan;LOU Yun;WANG Xin-yu;TAO Jing;ZHANG Yong(School of Public Health,Capital Medical University,Beijing 100069,China;Beijing Center for Disease Prevention and Control/Beijing Research Center for Preventive Medicine,Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing 100013,China)
出处
《毒理学杂志》
CAS
CSCD
2019年第2期96-102,共7页
Journal of Toxicology
基金
国家自然科学基金(81641184
81273108)
北京市公益院所科研项目(2017-19)
