期刊文献+

低氧暴露对大鼠肠道微生物群落的影响 被引量:14

Effects of hypoxic exposure on rats' gut microbiota
原文传递
导出
摘要 【背景】低氧暴露时机体会对物质的吸收和代谢作出调整,以维持自身的能量需求。肠道微生物参与了宿主众多生理过程,尤其在宿主消化、代谢、免疫等多方面发挥了重要作用。而目前对于低氧暴露过程中宿主肠道微生物群落结构和功能的动态变化,以及这些变化与宿主习服低氧之间的关系则少有报道。【目的】研究大鼠暴露于低氧环境后其肠道微生物群落的结构和多样性,探讨低氧暴露对宿主肠道微生物群落的影响,以及肠道微生物群落的变化与宿主代谢之间可能存在的关系。【方法】分别收集低氧暴露1、7、14、21和28 d的实验组(模拟海拔4 500 m)和对照组(43.5 m)的SD大鼠粪便样品,通过IlluminaHiSeq测序平台对样品进行16SrRNAV3-V4区测序,利用Uparse、Qiime、LEfSe和PICRUSt软件分析肠道微生物群落结构、丰度、多样性、组间差异,并利用KEGG数据库对肠道微生物的功能进行预测。【结果】不同低氧暴露时间组和对照组SD大鼠肠道微生物群落特征具有明显差异。低氧暴露组SD大鼠的肠道微生物群落中拟杆菌门(Bacteroidetes)、拟杆菌目(Bacteroidales)、拟杆菌科(Bacteroidaceae)、普氏菌科(Prevotellaceae)、普氏菌属(Prevotella)和普氏菌种(copri)的相对丰度较高且具有统计学意义。相应时间的对照组SD大鼠肠道微生物群落中瘤胃球菌科(Ruminococcaceae)、瘤胃球菌属(Ruminococcus)、梭菌纲(Clostridia)和梭菌目(Clostridiales)等相对丰度较高且具有统计学意义。功能预测发现遗传信息处理和代谢相关通路在低氧暴露组SD大鼠肠道微生物中明显富集。【结论】低氧暴露过程中SD大鼠肠道微生物群落结构和多样性呈动态变化,碳水化合物代谢相关菌群(普氏菌和拟杆菌)明显增加,可能参与了宿主能量代谢调整,有利于机体对低氧环境的习服。 [Background]Individuals adjust their nutrition absorption and energy metabolism when exposing to hypobaric hypoxia.Gut microbiota performs essential functions for host physiology,including digestive,metabolic,and immune mechanisms.However,little is known about the dynamic composition of gut microbiota as well as metabolic function during host acclimatized to hypobaric hypoxia.[Objective]To characterize and compare the gut microbiota of SD rats during 1,7,14,21 and 28 days after being exposed to a simulated altitude of 4 500 m(hypobaric chamber)with control groups(43.5 m).[Methods]We studied fecal samples by Illumina HiSeq platform targeting the V3-V4 region of the 16 S rRNA gene.Sequences were processed using the QIIME software package,UPARSE pipeline.LEfSe analysis was used to determine the different species between groups.The functional profiles of microbial communities were predicted by using PICRUSt.[Results]Fecal microbiota analysis revealed that the hypoxic exposure caused distinctive gut microbiota in rats,compared to the control groups.We detected the overrepresentation of Bacteroidetes,Bacteroidales,Bacteroidaceae,Prevotellaceae,Prevotella,copri and underrepresentation of Ruminococcaceae,Ruminococcus,Clostridia and Clostridiales in hypoxic exposure rats’feces.PICRUSt analysis revealed that Genetic Information Processing and metabolic related pathways were enriched in the hypoxic exposure groups than those in the control groups.[Conclusion]Hypoxic exposure caused dramatic changes of the structure,diversity and predicted function of gut microbiota in rats.The relative abundance of gut microbiota with complex glycan depolymerisation were enriched after hypoxic exposure.Those may involve in host adjustment of metabolism,and could be beneficial for host acclimatized to hypobaric hypoxia.
作者 马燕 马爽 尚春香 格日力 MA Yan;MA Shuang;SHANG Chun-Xiang;Gerili(Research Center for High Altitude Medicine,Qinghai University Medical College,Xining,Qinghai 810001,China;Key Laboratory for Application of High Altitude Medicine in Qinghai Province,Xining,Qinghai 810001,China)
出处 《微生物学通报》 CAS CSCD 北大核心 2019年第1期120-129,共10页 Microbiology China
基金 国家重点基础研究収展规划(973计划)(2012CB518200) 国家自然科学基金(31571231)~~
关键词 低氧 肠道微生物 高通量测序 Hypoxia Gut microbiota High-throughput sequencing
  • 相关文献

参考文献4

二级参考文献47

共引文献35

同被引文献125

引证文献14

二级引证文献29

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部