摘要
目的:对褐藻胶寡糖的食用安全性进行毒理学评价。方法:采用小鼠急性经口毒性试验、遗传毒性试验(Ames试验、小鼠骨髓细胞微核试验、小鼠精母细胞染色体畸变试验)和大鼠28天经口毒性试验。结果:小鼠急性经口半数致死剂量(LD50)大于15000 mg/kg BW;Ames试验、小鼠骨髓细胞微核试验、小鼠精母细胞染色体畸变试验结果均为阴性;在28天经口毒性试验中,大鼠的生长发育、血液学、血生化、尿常规及病理组织学检查未见与受试物相关的异常变化。结论:在试验剂量范围内,褐藻胶寡糖为实际无毒,无遗传毒性,28天经口毒性试验未发现明显毒性反应。
Objective:To assess the edible safety of alginate oligosaccharide.Methods:The toxicity of alginate oligosaccharide was investigated by acute oral test,genetic toxicity test including Ames test,mice marrow cell micronucleus test and mice spermatocyte chromosome aberration test,and 28-day oral toxicity test in rats.Results:The acute oral LD50(50%lethal dose)in mice was over 15000 mg/kg BW.No evidences of genotoxic activity to mice were foud.BY 28-day oral toxicity test,no significant changes in organ weight,hematologic,serum biochemical and urinalysis were noted,and no test substance-related pathological changes were found.Conclusion:No acute oral toxicity or genotoxicity of alginate oligosaccharide was found in this test dose range,and the toxicity of alginate oligosaccharide was not observed at the end of 28-day oral toxicity test.
作者
刘泳廷
郑冲
刘玲
叶建方
LIU Yong-ting;ZHENG Chong;LIU Ling;YE Jian-fang(Center for Disease Control and Prevention of Guizhou Province,Guiyang550004,China)
出处
《微量元素与健康研究》
CAS
2019年第3期5-8,共4页
Studies of Trace Elements and Health
关键词
褐藻胶寡糖
急性毒性
遗传毒性
28天经口毒性
安全性评价
alginate oligosaccharide
acute toxicity
Genotoxicity
28-day oral toxicity
Safety evaluation
