摘要
目的 获取 D1S5 4 9,D3S175 4 ,D12 S391,D12 S375和 D18S86 5基因座在中国康巴藏族群体中基因型及等位基因频率数据 ,初步探讨其在遗传学研究及法医学应用中的意义。方法 抽取 10 0名地区藏族群体无血缘关系个体的静脉血滴于滤纸上 ,干燥后剪取少许血痕 ,chelex法提取 DNA。应用 PCR技术 ,扩增产物用非变性聚丙烯酰胺凝胶垂直电泳分型。结果 中国康巴地区藏族群体 D1S5 4 9基因座发现 7个等位基因 ;D3S175 4基因座发现 6个等位基因 ;D12 S391基因座发现 10个等位基因 ;D12 S375基因座发现 6个等位基因 ;D18S86 5基因座发现 6个等位基因 ;5个基因座的基因型分布均符合 Hardy- Weinberg平衡 (P>0 .0 5 )。各基因座的杂合度分别为 0 .86 ,0 .77,0 .81,0 .6 9和 0 .80 ;个人识别率分别为 0 .92 9,0 .838,0 .934,0 .886和 0 .890 ;非父排除率分别为 0 .715 ,0 .5 4 5 ,0 .6 18,0 .4 13和 0 .5 99。结论 5个基因座在中国康巴藏族群体中具有较高的非父排除率与个人识别率 ,在法医学应用和群体遗传学研究中有较高的价值。
Objective To get preliminary genotype and allele frequency distributions of D1S549, D3S1754, D12S391, D12S375 and D18S865 loci in Chinese Zang(Tibetan) population in Kangba area and to validate more short tandam repeat(STR) systems for forensic application. Methods Fresh samples of venous blood from 100 unrelated individuals of Chinese Zang population in Kangba area were dropped on the filter paper. After they dried up, DNA were extracted by chelex-100 method and amplified by the polymerase chain reaction(PCR).The PCR products were analyzed by PAG vertical electrophoresis and silver staining. Results Seven alleles were found at D1S549 locus,six alleles at D3S1754 locus,ten alleles at D12S391 locus,six alleles at D12S375 and six alleles at D18S865 locus.No deviations from Hardy-Weinberg equilibrium were observed.The heterozygosities observed were 0.86,0.77,0.81,0.69,0.80 for D1S549,D3S1754,D12S391,D12S375 and D18S865 respectively. The calculated values of the Power of Exclusion were 0.715,0.545,0.618,0.413 and 0.599, and the values of the Power of Discrimination were 0.929,0.838,0.934,0.886 and 0.890, respectively. Conclusion All of the five loci in this study were useful for individual identification and paternity test, as well as for research purposes in genetics.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第1期21-24,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金 (批准号 3 0 1710 3 3 )资助
关键词
短串联重复序列
聚合酶链反应
遗传多态性
电泳
Short tandem repeat Polymerase chain reaction Genetic polymorphism Electrophoresis
