摘要
将黄芪多糖、当归多糖、蜂胶多糖、淫羊藿多糖、板蓝根多糖、黄芪黄酮、蜂胶黄酮、淫羊藿黄酮、黄芪皂甙、人参皂甙等 1 0种中药成分与鸡传染性法氏囊病毒 (IBDV)以 3种顺序加入到培养 2 4h的鸡胚成纤维细胞 (CEF)中。即先加中药后接种病毒、先接种病毒后加中药、中药和病毒同时加入 ,观察细胞病变的程度 ,以评价它们对IBDV感染细胞的影响。结果表明 ,先加中药后接种病毒和中药与病毒同时加入时 ,多数中药成分处理组在病毒接种后 72h的细胞病变程度明显减轻 ,表明多数中药能抑制病毒感染细胞 ,且有一定的量效和时效关系。
Ten Chinese herbal medicinal ingredients,astragalus polysaccharide,Chinese angelica polysaccharide,propolis polysaccharide,epimedium polysaccharide,isatis root polysaccharide,astragalus flavone,propolis flavone,epimedium flavone,astragalus saponin and ginsenosides,were added into chick embryo fibroblast(CEF)cell monolayer with infectious bursal disease virus(IBDV)at the same time or at different times respectively.The cell lesions were observed to evaluate the effects of ten ingredients on infection of the virus in the cell.The results showed that when the ingredients were processed early or simultaneously with IBDV, most Chinese herbal medicinal ingredients could decrease cytopathy and inhibit virus to infect the cell in dose-and time-dependent ways.
出处
《畜牧与兽医》
北大核心
2003年第12期8-10,共3页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金资助项目 (30 0 70 566)
