摘要
用中空纤维膜进行了从苦豆子盐酸提取物中纯化生物碱的超滤实验.研究了超滤和水连续稀释过滤对生物碱的纯化结果.同时考察了膜孔径的大小、操作压力、物料预处理和pH值对超滤效果的影响.实验结果表明:苦豆子盐酸提取物的超滤和稀释过滤能有效除去蛋白质和其他不纯物,透过液中总生物碱的回收率高.
Purification of the alkaloids by ultrafiltration using hollow fibre membrane from hydrochloric acid extract of Sophora alopecuroides L was investigated. Ultrafiltration followed by discontinuous diafiltration was carried out to examine the result of purification of alkaloids. Meanwhile, the effects of membrane pore size, operating pressure, pretreatment of the extract and pH value on ultrafiltration performances were also researched. The results showed that the ultrafiltration with discontinuous diafiltration could effectively remove proteins and other impurities. The recovery ratio of alkaloid from the permeate was high.
