摘要
【目的】研究毛橘红与光橘红石油醚部位指纹图谱差别。【方法】取毛橘红和光橘红各十二个样品,应用紫外光谱法和薄层包谱法对其化学成分进行检测。【结果】薄层色谱检测表明,光橘红中能检测到是淡绿黄色斑点(即特异点),而毛橘红未能检测出。紫外光谱检测显示,毛橘红有6个共有峰,其峰值比为2:1.5:1.5:1:0.9:1;光橘红只有4个共有峰,其峰值比为2.8:1:1:1。说明毛橘红与光橘红的石油醚部位在紫外光谱指纹图谱和薄层色谱指纹图谱上均存在较大差别。【结论】紫外光谱指纹图谱和薄层色谱指纹图谱均可以作为简便、快速、稳定区别毛橘红与光橘红的指标。
【Objective】To compare the fingerprints of petroleum ether extractive from Citrus grandis Osbeck. Var.
tomentosa Hort. (CGO Var. TH) and Citrus grandis (L.) Osbeck (CGO).【Methods】Thin-layer chromatography
(TLC) and ultraviolet (UV) spectrometry were adopted. 【Results】TLC showed light-green yellow spots (specific
spots) were found in Citrus grandis (L.) Osbeck and were undetectable in Citrus grandis Osbeck. Var. tomentosa
Hort. . Ultra-violet spectrometry showed that 6 peaks were shared in Citrus grandis Osbeck. Var. tomentosa Hort. and
their ratio was 2:1.5:1.5:1:0.9:1, and 4 peaks in Citrus grandis (L.) Osbeck and their ratio was 2.8:1:1:1. There
were great differences between the fingerprints detected with TLC and those with UV spectrometry.【Conclusion】TLC
and UV spectrometry are efficient and stable. Both UV-fingerprints and TLC fingerprints can be used for the identification
of Citrus grandis Osbeck. Var. tomentosa Hort. and Citrus grandis (L.) Osbeck.
出处
《广州中医药大学学报》
CAS
2003年第4期305-307,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省科技计划项目(编号:A301020101)
关键词
毛橘红
光橘红
紫外光谱
薄层色谱
指纹图谱
化学成分
EXOCARPIUM CITRI GRANDIS/analysis
C HROMATOGRAPHY,THIN LAYER
SPECTROPHOTOMETRY, ULTRAVIOLET
