摘要
通过PCR技术克隆了极大螺旋藻藻蓝蛋白基因,构建了克隆载体和表达载体,并将该基因导入巴斯德毕赤酵母X 33基因组,筛选了能够有效表达藻蓝蛋白基因的毕赤酵母工程菌株PC8,从而为发酵法生产藻蓝蛋白奠定了基础.
The phycocyanin gene from Spirulina maxima was cloned by PCR.The clone vector and the expression vector were constructed,respectively.The latter was transformed into Pichia pastoris X-33 genome and transformant PC8 that can efficiently express the phycocyanin gene was screened,which lays foundation for the production of the recombinant phycocyanin by fermentation technology.
出处
《科技通报》
北大核心
2004年第1期21-23,27,共4页
Bulletin of Science and Technology
基金
浙江省科学技术厅研究基金(Z40165)
浙江省教育委员会研究基金资助项目(纵Y01-42)
