摘要
采用MRS培养基,模拟人体胃酸环境(pH1.5～4.5)和十二指肠高胆汁盐环境(1～4g/kg)对嗜酸乳杆菌的抗性进行了研究。结果表明,嗜酸乳杆菌在pH2.5～4.5时具有较强的生存能力,37℃培养6h活菌数仍达107mL-1以上,pH1.5条件下仍有部分存活。在1～3g/kg胆汁盐条件下培养4h,活菌数仍达106mL-1以上,且能在4g/kg胆汁盐中存活。回归分析的F检验值达极显著水平。
Using MRS medium,the intolerance ability of Lactobacillus acidophilus was studied imitating gastroenteric environments (pH 1.5-4.5 and 1-4 g/kg bile salt).The results showed that the survival ability of L.acidophilus was higher at pH 2.5-4.5,the living bacterial number was above 10~7 cfu/mL after 6 h culture at 37 ℃,and some L.acidophilus was still alive at pH 1.5;that the living bacterial number was above 10~6 cfu/mL at 1-3 g/kg bile salt after 4 h culture and some bacteria could survive at 4 g/kg bile salt.The F inspection value was extremely remarkable by regression analysis.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2004年第2期57-60,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
河南职业技术师范学院重点科研项目(02010505)
关键词
嗜酸乳杆菌
抗酸能力
抗胆汁盐能力
人体胃酸环境
存活能力
Lactobacillus acidophilus
gastroenteric environment
intolerance ability
regression analysis
