摘要
目的 通过研究人高、低转移肺大细胞癌细胞系基因差异表达谱 ,克隆与肿瘤转移相关的基因。方法 应用mRNA差异显示技术 (DDRT PCR)对高、低转移人肺大细胞癌细胞系的mRNA进行差异显示 ,切胶回收差异表达cDNA ,克隆测序后与GenBank数据库进行Blast比对。结果 获得差异表达cDNA 5 0条 ,其中已知功能基因 18条 ,未知功能基因 16条 ,DNA来源 16条 ,EST 4条。结论 人高、低转移肺大细胞癌细胞系的基因表达差异较大 ,转移的过程受基因的表达与调控的影响。应用DDRT PCR技术有望找到调控肿瘤转移的基因 。
Objective To investigate the differentially expressed gene profile between high rate and low rate metastatic large cell lung cancer cell lines via differential display reverse transcript PCR (DDRT PCR). Methods DDRT PCR was performed in two large cell lung cancer cell lines with different metastatic ability. The differentially displayed cDNA were cloned and sequenced and compared with the sequences in the GenBank.Results Fifty differentially displayed cDNAs were acquired, including 18 function known genes, 16 function unknown genes, 16 genomic DNA fragments and 4 ESTs. Conclusion The cell lines have different gene expression profiles, and metastasis is affected by multi gene expression and regulation. Some genes which contribute to the metastasis of lung cancer could be found by DDRT PCR, and this study gives some new clues to cancer diagnosis and therapy.
出处
《中国肺癌杂志》
CAS
2003年第5期347-351,共5页
Chinese Journal of Lung Cancer
基金
国家自然科学基金项目 (30 0 70 335)资助~~
