摘要
目的:探讨手足口病的实验室检测以及相关辅助诊断方法。方法:收治手足口病患者32例,采用实时荧光PCR方法检测手足口病的病原菌,并与常规RT-PCR方法检测结果进行比较。结果:肠道病毒、肠道病毒71型、柯萨奇病毒A16型为手足口病常见的病原体,两种检测结果比较差异无统计学意义(P>0.05)。结论:实时荧光PCR检测方法与常规RT-PCR方法检测吻合度较高,肠病毒71型可作为早期诊断手足口病的指标之一。
Objective:To explore the laboratory detection and related assistant diagnosis method of hand foot mouth disease.Methods:32 patients with hand foot mouth disease were selected.Using real-time fluorescence PCR method detected the pathogeny of hand foot mouth disease.The results were compared with the detection results of conventional RT-PCR method.Results:The intestinal virus,enteric virus type 71,coxsackie virus A16 type are the common pathogens of hand foot mouth disease.There was no significant difference between the two detection results(P>0.05).Conclusion:The real-time fluorescence PCR detection method and conventional RT-PCR method have higher detection fitness.The intestinal virus could be used as an indicator for early diagnosis of hand foot mouth disease.
出处
《中国社区医师》
2015年第25期114-115,共2页
Chinese Community Doctors
关键词
手足口病
实验室检测
辅助诊断
Hand foot mouth disease
Laboratory detection
Assistant diagnosis
