摘要
目的观察神经病理性疼痛发病早期0.25 MPa高压氧治疗对大鼠疼痛行为学的影响,并探讨早期治疗的作用机制。方法采用左后肢坐骨神经结扎术建立慢性压迫性损伤大鼠模型,术后早期(第1天)即开始进行高压氧(0.25 MPa)治疗(60 min/d),5d后观察大鼠一般情况、自发缩足次数、缩足阈值(PWT)和缩足潜伏期(PWL)等疼痛行为学变化。结果与假手术组相比,模型组大鼠体质量显著下降(t=4.772,P=0.000)、高压氧组大鼠体质量降低幅度小于模型组(t=2.411,P=0.029);模型组大鼠术后即出现缩足潜伏期缩短(t=28.345,P=0.000),第3天开始自发缩足次数增多(t=12.541,P=0.000)、缩足阈值降低(t=4.032,P=0.001)。与模型组相比,高压氧治疗组大鼠术后第3天开始缩足次数减少(t=8.077,P=0.000)、缩足阈值增加(t=2.114,P=0.049)、缩足潜伏期延长(t=7.715,P=0.000)。结论早期施行高压氧(0.25 MPa)治疗可以显著改善神经病理性疼痛大鼠痛敏症状,为临床神经病理性疼痛提供一种新的便捷、经济、有效的治疗方法。
Objective To observe the effect of early use of 0.25 MPa hyperbaric oxygen(HBO) on pain behavior in mo del rat of neuropathic pain(NP) an d explore the mechanism.Metho dsFifty four male Sprague Dawley(SD) rats were ran domly divi de d into 3 groups(18 rats per group) inclu ding sham operation, sciatic nerve ligation with chronic constriction injury(CCI) an d HBO early treatment groups. The rat mo del of neuropathic pain was establishe d, an d CCI + HBO group receive d 0.25 MPa HBO treatment for 60 min per day at early stage, continuing for 5 d. The changes in general state of health, the number of paw with drawal responses, paw with drawal threshol d(PWT) an d paw with drawal latency(PWL) were assesse d at different time points after operation.ResultsCompare d with sham operate d group, the bo dy weight of rats in CCI group decrease d significantly(t=4.772, P=0.000) on the 14th day; the bo dy weight of rats in CCI + HBO group was significantly higher than that in CCI group(t = 2.411, P = 0.029). Compare d with sham operate d group, re duce d PWL imme diately after operation(t = 28.345, P = 0.000), an d more paw with drawal responses(t=12.541, P=0.000) an d decrease d PWT(t=4.032, P=0.001) from the 3r d day after operation were observe d in CCI group. Compare d with CCI group, the number of paw with drawal responses decrease d(t = 8.077, P = 0.000), an d PWT an d PWL increase d(t = 2.114, P = 0.049; t = 7.715, P = 0.000) in CCI + HBO group.Conclusion 0.25 MPa HBO treatment at early stage can ameliorate the mechanical an d thermal hyperalgesia. HBO may provi de a new convenient an d effective way for the treatment of neuropathic pain in clinic.
出处
《中国现代神经疾病杂志》
CAS
2013年第9期766-771,共6页
Chinese Journal of Contemporary Neurology and Neurosurgery
基金
辽宁省沈阳市科学技术项目计划(项目编号:F10-205-1-41)~~
关键词
神经痛
创伤和损伤
坐骨神经
高压氧
疾病模型
动物
Neuralgia
Wounds and injuries
Sciatic nerve
Hyperbaric oxygenation
Disease modals,animal
