摘要
目的建立UPLC测定中成药和保健食品中16种非法添加的抗过敏类药物。方法采用WatersBEHC18色谱柱(100 mm×2.1mm,1.7μm),以甲醇-20mmol·L-1磷酸二氢钠(用磷酸调节pH值至3.0)为流动相,梯度洗脱,检测波长为220 nm。结果 16种抗过敏类药物在2.5～40μg·mL^(-1)内呈良好线性关系(r2>0.999),平均回收率为90.77%～113.35%,RSD为0.06%～1.21%;定量限为0.18～3.92μg·mL^(-1),检出限为0.09～0.78μg·mL^(-1)。结论该方法简便、灵敏度高,可有效地判断中成药及保健品中是否非法掺入16种抗过敏类药物。
OBJECTIVE To develop a UPLC method to determine 16 anti-allergic drugs illegally added in Chinese traditional patent medicine and health food. METHODS The separation was performed on a Waters BEH C18 column(100 mm×2.1 mm, 1.7 μm) with the mobile phase consisted of methanol-20 mmol·L-1 sodium dihydrogen phosphate solution(adjusted to pH 3.0 with phosphoric acid) in a gradient elution mode, the detector wavelength was 220 nm. RESULTS The results of 16 anti-allergic drugs showed good linearity in the range of 2.5-40 μg·mL-1(r2>0.999). The average recoveries were from 90.77% to 113.35%, with the RSD from 0.06% to 1.21%. The limits of quantifications were among 0.18-3.92 μg·mL-1, and the limits of detections were among 0.09-0.78 μg·mL-1. CONCLUSION This method is simple and sensitive, and it can effectively judge whether the Chinese patent medicines and health products are illegally mixed with these 16 anti-allergic drugs.
作者
金朦娜
彭彦
王秀秀
汪霞
杨直
JIN Mengna;PENG Yan;WANG Xiuxiu;WANG Xia;YANG Zhi(Hangzhou Institute for Food and Drug Control,Hangzhou 310022, China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2019年第2期186-190,共5页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省食品药品监管系统科技计划项目(BH201601)
关键词
超高效液相色谱
抗过敏药物
非法添加
中成药
保健品
UPLC
anti-allergic drugs
illegal addition
Chinese traditional patent medicine
health foods
