摘要
旨在为猪支原体肺炎灭活疫苗研发水性的或水包油型佐剂,降低疫苗的副作用。利用小鼠为模型动物评价几种不同佐剂配合猪支原体肺炎灭活疫苗免疫动物时对细胞免疫和体液免疫应答的增强作用。将不同佐剂与灭活疫苗配合肌肉注射免疫小鼠,检测脾淋巴细胞对猪肺炎支原体抗原的增殖应答情况及特异性的血清IgG抗体水平。试验分两批次进行,共评价11种佐剂。结果表明,卡波姆974+免疫刺激复合物基质混合物佐剂和壳聚糖+左旋咪唑混合物佐剂强烈地诱导产生高水平细胞免疫和体液免疫应答(P<0.01);卡波姆974+左旋咪唑混合物佐剂、卡波姆974+左旋咪唑+黄芪多糖混合物佐剂和ISA 11R VG佐剂次之(P<0.05);β-葡聚糖+左旋咪唑混合物佐剂、DEAE-dextran+左旋咪唑+黄芪多糖混合物佐剂和β-葡聚糖+左旋咪唑+黄芪多糖混合物佐剂在细胞免疫应答方面作用显著(P<0.01),但体液免疫应答作用稍弱;而GEL 01ST佐剂在体液免疫应答方面作用显著(P<0.01),细胞免疫应答作用稍弱。通过分析认为卡波姆974+免疫刺激复合物基质混合物佐剂、壳聚糖+左旋咪唑混合物佐剂的效果最好,可同时显著增加细胞及体液免疫应答,本试验结果将为猪支原体肺炎灭活疫苗的佐剂研发提供数据基础。
The purpose of this study was to develop a novel aqueous or oil-in-water adjuvant for the Mycoplasma hyopneumoniae inactivated vaccine as well as to reduce the side effect of the vaccine.The Kunming mice were used as the animal model to evaluate the immune enhancement effect of different adjuvants to the inactivated vaccine.After immunization,the response of lymphocyte proliferation and the production of serum antibody IgG were evaluated.The experiments were carried out as two individual parts and 11 kinds of adjuvants were tested in total.The results indicated that the carbomer974+ ISCOM-matrix mixture adjuvant and the chitosan +levamisolemixture adjuvant strongly induced high levels of cellular immune and humoral immune response,better than the effect of the carbomer974 + levamisole mixture adjuvant,the carbomer974+ levamisole+ astragalus polysaccharides mixture adjuvant and the ISA 11 RVG adjuvant.Animals of the groups of the beta- glucan+levamisole mixture adjuvant,the DEAE-dextran+levamisole+astragalus polysaccharide mixture adjuvant and the beta- glucan+levamisole+ astragalus polysaccharide mixture adjuvant had obvious cellular immune response,but weak humoral immune response.Additionally,animals of the group of the GEL 01 ST adjuvant had obvious humoral immune response,but weak cellular immune response.The carbomer974+ISCOM-matrix mixture adjuvant and the chitosan+levamisole mixture adjuvant had the best effect on both cellular and humoral immune response.This data would help in the subsequent research on the inactivated vaccine against Mycoplasma hyopneumoniae.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第12期2028-2033,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省农业科技自主创新资金项目(cx(13)3066)
关键词
水性佐剂
水包油佐剂
猪支原体肺炎灭活疫苗
细胞免疫
体液免疫
acquous adjuvant
oil-in-water adjuvants
Mycoplasma hyopneumoniaeinactivated vaccine
cellular immune response
humoral immune response
