摘要
目的 研究电针治疗对脑梗死病灶周围及海马神经元特异性烯醇化酶 (NSE)及胶质纤维酸性蛋白 (GFAP)表达的影响。方法 采用易卒中型肾性高血压大鼠 (RHRSP) ,电凝法凝闭大脑中动脉 (MCAO)。MCAO后给予电针治疗 ,在治疗 5d、2周后分别处死 ,行免疫组化染色 ,计算NSE、GFAP的阳性单位值。结果 梗死边缘NSE表达在梗死 5d时处于低水平 ,梗死 2周组增高 ,电针治疗 2周后海马的NSE表达明显增加 ;梗死 5d时病灶周围GFAP阳性细胞数目增多 ,染色加深 ,胞体增大 ,突起增粗、增长 ,2周时变化更明显。电针治疗 5d后 ,梗死边缘GFAP阳性单位值明显增高。结论 早期电针治疗能够提高海马区NSE及梗死灶周围GFAP的表达 ,促进了缺血后脑的可塑性变化 ,构成了脑梗死功能恢复的物质基础。
Objective To investigate the effect of electroacupuncture(EA) on neuron-specific enolase(NSE) and glial fibrillary acid protein (GFAP) expression of ischemic perifocal area and hippocampus in brain of rats after acute cerebral infarction. Methods Stroke-prone renovascular hypertensive rats(RHRSP) were used. Cerebral infarction was established with middle cerebral artery occlusion(MCAO) caused by electric coagulation. EA was applied 24 hour after cerebral infarction for 5 days a week. Neurobehavioral tests and scoring of these tests were carried out once a day (twice a week the next week) starting on the day after MCAO and continuing until the end of each experiment by Garcia test. The rats were sacrificed after EA for 2 weeks. The NSE and GFAP positive cells in ischemic perifocal area, contralateral mirror area and the hippocampus were examined by immunohistochemistry staining. The positive unit (PU) of immunohistochemistry staining cells was calculated by Microscope-computer colour image analysis. Results The Garcia scores were less than 18 until the 5th day after MCAO,and the scores in EA group were significantly higher than those in the control group at the second and third days. The PU of NSE in ischemic perifocal area was low at the 5th day after MCAO, and it increased significantly in ischemic perifocal area (P=0.05) and hippocampus(P<0.05) 2 weeks later. These index was higher in hippocampus than in the corresponding region of control (P=0.04), contralateral(P=0.02),sham operation groups(P=0.01) and 5d treatment group(P=0.01) after 2 weeks of EA treatment. At the 5th day after MCAO,the number of GFAP immunopositive cells enhanced, their staining strong,the cell bodies enlarged and the neurites long and thick. Two weeks later,these changs became obvious and the PU of GFAP was higher in ischemic perifocal area than that in the corresponding region of contralateral(P=0.02),sham operation group(P=0.02) and MCAO 5d group(P=0.03).The PU of GFAP was significantly increased after EA treatment(P=0.03) and this condition existed until the end of 2 weeks. Conclusion EA may increase expression of NSE in the hippocampus and GFAP in the ischemic perifocal area and can promote the neuronal plasticity in the early stage of ischemia. The relevant changes may form the material basis of functional recovery after ischemia.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2003年第12期708-712,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
原中山医科大学"2 1 1工程"课题基金资助项目 (No .0 61 )
