摘要
目的 :了解妇科感染性疾病患者生殖道中肺炎支原体、生殖支原体、发酵支原体、穿通支原体和梨支原体的感染情况 ,为临床诊治提供科学依据。方法 :采集妇科感染性疾病患者和正常女性 (各 172例 )的宫颈管分泌物 ,接种于SP 4培养基作分离培养。阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA测序来加以鉴定。结果 :172例患者的宫颈分泌物中分离到肺炎支原体 2株 (1 16 % ) ,生殖支原体 4株 (2 33% ) ,发酵支原体 2株(1 16 % ) ,穿通支原体 1株 (0 5 8% )。而 172例正常女性未分离到相应菌株。结论 :妇科感染性疾病患者生殖道中存在肺炎支原体、生殖支原体、发酵支原体和穿通支原体感染 ,在临床诊治中应予关注。
Objective: To understand infectious state of five kinds of mycoplasmas--Mycoplasma pneumoniae (Mp), Mycoplasma gentalium (Mg), Mycoplasma menetrans (Mpe), Mycoplasma fermentans (Mf), and Mycoplasma pirum (Mpi) in patients with female genital infection, and to provide some information for clinical diagnosis and therapy. Methods: The cervical secretions were collected from 172 patients and 172 healthy controls, and the samples were inoculated into modified SP-4 culture medium for isolating. Meanwhile, biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR), and DNA sequence assay were employed to identify those positive cultures. Results: There were two strains of Mp (1.16%), four strains of Mg ( 2.33%), two strains of Mf (1.16%) and one strain of Mpe (0.58%) being isolated from samples of 172 patients with genital infection. But none was found in healthy controls. Conclusions: The study confirmed that there exist Mp, Mg, Mpe, and Mf in reproductive tract in patients with female genital infection. We should pay more attention in clinical works.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2003年第6期350-351,共2页
Journal of Practical Obstetrics and Gynecology
