摘要
目的 建立氯化锂 匹罗卡品化学诱导的大鼠急性癫痫模型。方法 雄性Wistar大鼠 55只 ,随机分为生理盐水对照组、地西泮组和致痫组。氯化锂腹腔注射后 10～ 10h后给予匹罗卡品。结果 生理盐水对照组大鼠均为 0级发作 ,地西泮组中 8只大鼠为 0级发作 ,2只出现Ⅰ级发作 ;致痫组均达到Ⅲ级以上的痫性发作 ,其中Ⅲ级 2只 (2 / 3 5) ,Ⅳ级 3只 (3 / 3 5) ,Ⅴ级 3 0只 (3 0 / 3 5)。致痫组大鼠在匹罗卡品腹注射后 10～ 90min内全部出现急性痫性发作。结论 氯化锂 匹罗卡品诱导的大鼠急性癫痫模型具有制作方便、致痫成功率高和动物死亡率低等特点 。
Objective To establish the lithium-pilocarpine induce d rat model of acute epilepsy.Methods 55 Wistar male rats were divided into the mormal saline control group,diazepam group and seizure-induced group randomly.Pilocarpine was injected intraperitoneally(ip) after the 10～18 hours of the lithium chloride ip.Results No seizure was found i n the normal saline control group,and 8 rats were stage 0 attack,2 rats were sta ge Ⅰ in diazepam group.In seizure-induced group,all was above stage Ⅲ,2 rats were stage Ⅲ(2/35),stage Ⅳ in 3 rats(3/35),stage Ⅴ in 30 rats(30/35).All o f the seizure-induced rats has presented acute epileptic attack after pilocarpi ne intraperitoneal injection within 10～90 minutes.Conclusion Li thium-pilocarpine-induced rats model of acute epilepsy was characterized easy building,with high successful rate and lower mortality of the animal;This model has mimic features of human status epilepticus and temporal epilepsy in seizure behavior and electroencephalographic findings.
出处
《中国血液流变学杂志》
CAS
2003年第4期308-309,312,共3页
Chinese Journal of Hemorheology
关键词
氯化锂
匹罗卡品
大鼠
急性癫痫模型
Lithium-pilocarpine
status epilepticus
rat
acute epi leptic model
