摘要
[目的]探讨体外培养定向诱导大鼠骨髓间充质干细胞(MSCs)向心肌样细胞分化的潜能。[方法]取大鼠下肢骨骨髓,分离培养MSCs,用5-氮胞苷(5-aza)定向诱导24 h,相差显微镜下观察其形态变化,免疫组化法检测肌钙蛋白(cTnT)和心肌细胞结蛋白(desmin)表达,并通过RT-PCR对肌球蛋白重链(MHC)在细胞中的表达进行鉴定。[结果]MSCs经5-aza诱导后,部分细胞呈梭形,并可见肌管样结构。免疫组化检测示诱导后MSCs的cTnT阳性细胞数为15％,Desmin表达强阳性,RT-PCR示诱导后MSCs有MHC表达。[结论]MSCs在体外能在一定条件下被诱导分化为心肌样细胞,是心肌缺血干细胞移植的较理想的细胞来源。
[Objective] To study the potential of rat mesenchymal stem cells ( MSCs ) to differentiate into cardiomyocytes by induction in vitro. [Methods] SD rat MSCs were isolated and cultured from rat bone marrow, then induced by 5-aza for 24 h. The MSCs differentiation was observed by phase-contrast microscope. The immunohistochemical technique was used for the expression of cTnT and desmin. The expression of myocin heavy chain ( MHC ) was examined by RT-PCR. [Results] After being induced by 5-aza, some of MSCs became spindle-like and formed myotube-like structure. And cTnT positive cells were about 15%, desmin positive cells were found by immunohistochemistry staining. The expression of MHC ( one of cardie-special genes ) could be detected by RT-PCR. [Conclusion] The MSCs can be differentiated to cardiomyocytes in vitro by 5-aza, and MSCs might be a new cell source of stem cell transplantation in ischemic myocardium.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2004年第1期67-69,76,共4页
Journal of Sun Yat-Sen University:Medical Sciences
