摘要
健康Wister雄性大鼠连续 6周腹腔内注射D 半乳糖 ,双侧海马内注射Aβ1-40 造成老年痴呆 (AD)模型。以Y 型迷宫实验检测大鼠的学习记忆行为 ,测定大脑皮层脂褐素含量以及海马线粒体膜流动性、SOD活性、MDA含量 ,电镜观察海马超微结构 ,了解观察褪黑素及红景天素对老年性痴呆模型大鼠的治疗作用。结果显示 ,褪黑素、红景天素均能显著改善痴呆大鼠的学习记忆障碍 ,使大鼠在Y 型迷宫中的训练次数减少 ,对照组 (C组 )、痴呆组 (D组 )、红景天治疗组 (R)组、褪黑素治疗组 (M组 )及联合治疗组 (RM组 )达到学会标准的训练次数分别是 2 7 92± 7 2 9,39 14± 12 35 ,2 8 15± 10 34,2 9 78± 15 74和 2 6 71± 14 70 ,明显逆转痴呆大鼠海马SOD活性的代偿性升高 (R组、M组、RM组中SOD分别降低 4 3 8%、6 1 2 %、5 8 2 % )、减少MDA含量及皮层脂褐素含量 (R组、M组和RM组中MDA分别降低 2 5 9%、2 9 6 %、5 8 5 % ;脂褐素含量减少百分数分别为 36 %、32 %和 5 2 % ) ,防止海马神经元的变性及凋亡。结果提示 ,褪黑素、红景天素治疗后的大鼠与痴呆组相比 ,行为障碍明显改善 ,自由基含量显著下降 ,海马锥体细胞的变性和凋亡现象明显好转。提示两种药物对AD模型大鼠均有一定的防治作用 。
D-galactose was intraperitoneally injected into rats for 6 weeks and Aβ 1-40 was injected into bilateral hippocampus to creat an AD model. Melatonin or Rhodosin was intraperitoneally injected in rats for 4 weeks to determine the protective and therapeutic effects in rats with AD. Y-maze test was used to determine the learning ability and memory of the rats. The content of lipofuscin in the central cortex, the viscous coefficient of mitochondrial membrane, the activity of superoxide dismutase and the content of malondialdehyde in bilateral hippocampus were determined. The apoptosis of hippocampus neurons was examined by electron microscopy. The result revealed that melatonin and Rhodosin as antioxidant significantly improved learning and memory of rats with AD(P<0.05) and increased SOD, MDA, the viscous coefficient and lipofuscin to their normal levels(P<0.05). The treatment also has protective effects on the neuronal cells from apoptosis. These results indicated that Melatonin and Rhodosin had preventive and therapeutic effects on rats with AD probably by reducing free radicals in rats but there was no synergistic action of the two substances.
出处
《基础医学与临床》
CSCD
北大核心
2003年第6期620-624,共5页
Basic and Clinical Medicine
基金
江苏省自然科学基金 (KJB310 0 0 4 )
