摘要
目的 研究中药活性成分柠檬醛和肉桂醛的抗曲霉菌活性 ,探讨其作用机制 ,为抗真菌中药进一步研究和开发提供实验依据。方法 采用微量液体稀释法测定柠檬醛、肉桂醛对黄曲霉菌和烟曲霉菌的最低抑菌浓度(MIC) ;运用同位素掺入技术测定在柠檬醛、肉桂醛作用下 ,黄曲霉菌和烟曲霉菌对3 H TdR ,3 H UdR和3 H 亮氨酸掺入的抑制率。结果 柠檬醛对黄曲霉菌、烟曲霉菌的MIC分别为 :2 .5 90 μg/ml、0 .6 4 8μg/ml,肉桂醛对黄曲霉菌、烟曲霉菌的MIC分别为 :0 .10 9μg/ml、0 .0 5 5 μg/ml;柠檬醛和肉桂醛能明显抑制三种同位素前体的掺入 (P <0 .0 1)。结论 柠檬醛和肉桂醛在体外有较高的抗曲霉菌活性。推测其抗真菌的作用机理可能是通过抑制曲霉菌的DNA、RNA、蛋白质的合成而表现杀菌效应。
Objective To study the antifungal activities of cinnamldehyde and citral in Aspergilli and to explore their antifungal mechanism in an attempt to provide experimental evidence for further application of anti Aspergilli medicinal herbs.Methods The minimum inhibitory concentration(MICs)of cinnamldehyde,citral against A.fumigatus,and A.flavus were determined by broth microdilution method;By means of incorporation experiment of isotope labelled precusor,the inhibitory rates of 3H TdR, 3H UdR,and 3H leucine which would be incorporated into the Aspergilli were measured after treatment with cinnamldehyde and citral respectively.Results MIC of cinnamldehyde against A.fumigatus and A.flavus were 0.109 μg/ml and 0.055 μg/ml respectively;MIC of citral against A.fumigatus and A.flavus were 2.590 μg/ml and 0.648 μg/ml respectively;Cinnamldehyde and citral could obviously inhibit incorporation of 3H TdR, 3H UdR and 3H leucine into Aspergilli( P <0.01).Conclusion The anti aspergilli mechanism of cinnamldehyde and citral may be involved with the inhibition of DNA, RNA and protein synthesis.
出处
《江西医学院学报》
2003年第6期10-13,共4页
Acta Academiae Medicinae Jiangxi
基金
国家自然科学基金资助 (NO :30 16 0 0 99)
关键词
柠檬醛
肉桂醛
曲霉菌
作用机制
中药
cinnamldehyde
citral
aspergilli
isotope incorporation
antifungal mechanism
