摘要
目的:观察转化生长因子(transforminggrowthfactor-β,TGF-β)、透明质酸和4-硫酸软骨质(chondroitin-4-sulfate,Ch-4-S)对增生性瘢痕(hypertrophicscar,HTS)成纤维细胞胶原酶和金属蛋白酶组织抑制因子(tissueinhibitorofmetalloproteinase-1,TIMP-1)表达的影响,探讨它们在HTS形成中的作用。方法:应用组织块培养法培养成纤维细胞,用原位杂交检测胶原酶和TIMP-1的表达。结果:TGF-β抑制胶原酶表达,其阳性细胞百分率为36%,明显低于对照组(t=4.52,P<0.05)。TGF-β促进TIMP-1的表达,其阳性细胞百分率为69.25%,明显高于对照组(t=3.62,P<0.05)。透明质酸上调胶原酶的表达,阳性细胞百分率为72.75%,显著高于对照(t=5.86,P<0.01)。结论:TGF-β抑制胶原酶表达,阻止胶原降解可能是增生性瘢痕形成的重要原因。透明质酸促进胶原酶表达,减轻瘢痕形成。
AIM:To observe the effects on the expression of mRNA for collagenase(MMP 1) and tissue inhibitor of metalloproteinase 1(TIMP 1) in fibroblasts from hypertrophic scar(HTS) by transforming growth factor β(TGF β),hyaluronic acid(HA) and chondroitin 4 sulfate(Ch 4 s),and to investigate their functions of HTS formation. METHODS:Fibroblasts were cultured by tissue block culture method and the expression of MMP 1 and TIMP 1 was detected by in situ hybridization and quantitative analysis were used in this study. RESULTS:The expression of mRNA for MMP 1 in fibroblasts was apparently decreased in cultures treated with TGF βand its positive cells percentage of 36%was obviously lower than that in the control group(t=4.52,P< 0.05),whereas the expression of TIMP 1 was greatly increased and its positive cells percentage of 72.75%was obviously higher than that in the control group(t=3.62,P< 0.05).HA increased the expression of MMP 1 in fibroblasts from HTS. CONCLUSION: TGF βcan inhibit the expression of MMP 1.The inhibition of collagen degradation may play an important role in the development of HTS.HA can relief the formation of HTS by increasing the expression of MMP 1.
出处
《中国临床康复》
CSCD
2003年第32期4316-4317,共2页
Chinese Journal of Clinical Rehabilitation
