摘要
目的探讨^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠MDA-MB231乳腺癌早期放疗疗效的价值。方法建立16只裸鼠MDA-MB231乳腺癌模型,将其按照随机对照原则分为两组:对照组(A组)、放疗组(B组),每组8只。每组裸鼠行micro PET/CT显像,测定每只裸鼠肿瘤SUVmax值。完成显像后,常规HE染色观察每组肿瘤组织形态学特征,免疫组化方法测定每组肿瘤细胞乏氧诱导因子-1α(HIF-1α)的表达情况。结果放疗前,对照组与放疗组SUVmax值差异无统计学意义(t=0. 375,P> 0. 05)。放疗组放疗后48 h裸鼠肿瘤组织SUVmax值较放疗前(t=9. 958,P <0. 05)、放疗后24 h(t=16. 506,P <0. 05)明显降低,差异有统计学意义(F=58. 860,P <0. 05)。放疗后24 h SUVmax值也低于放疗前24 h(t=5. 405,P <0. 05),差异有统计学意义。HE染色结果显示放疗组肿瘤细胞坏死较对照组更加明显。免疫组化结果显示放疗组放疗后HIF-1α表达阳性率明显低于放疗前(t=14. 802,P <0. 05),差异具有统计学意义。相关性分析结果显示肿瘤SUVmax与HIF-1α的表达呈明显正相关性(r=0. 865,P <0. 05)。结论^(18)F-氟赤硝基咪唑micro PET/CT可以监测肿瘤内部的乏氧状态,并且可以评价裸鼠MDA-MB231乳腺癌的早期放疗疗效。
Objective To investigate the value of18F-FETNIM micro PET/CT in assessing the radiotherapeutic efficacy in MDA-MB231 breast cancer model. Methods Sixteen MDA-MB231 breast cancer models were randomly diveded into two groups(8 rats in each group):control group( group A) and radiotherapy group( group B).18FFETNIM micro PET/CT imaging was performed in two groups to measure the SUVmax of tumor in all nude mice.The morphological features of tumor tissue in each group were observed by HE staining. The expression of hypoxia inducible factor 1α( HIF-1α) in two groups were determined by immunohistochemistry. Results There was no significant difference in the SUVmax value between control group and radiotherapy group before radiotherapy( t =0. 375,P > 0. 05). The SUVmax value of tumor tissue when 48 h after radiotherapy was significantly lower than that before radiotherapy( t = 9. 958,P < 0. 05) and 24 h after radiotherapy( t = 16. 506,P < 0. 05),the difference was statistically significant( F = 58. 860,P < 0. 05). The SUVmax value of tumor tissue when 48 h after radiotherapy was also lower than before radiotherapy( t = 5. 405,P < 0. 05). More obvious necrosis was observed in the radiotherapy group than the control group by HE staining. The expressions of HIF-1α in the radiotherapy group were significantly decreased compared with the control group( t = 14. 802,P < 0. 05). The tumor SUVmax value was positively correlated with HIF-1α expression( r = 0. 865,P < 0. 05). Conclusion 18F-FETNIM micro PET/CT not only can be used to detect the hypoxic state within the tumor,but also can evaluate the early radiotherapeutic efficacy in MDA-MB231 breast cancer in nude mice.
作者
苏晓雨
徐慧琴
汪会
余文静
张丹
谯凤
Su Xiaoyu;Xu Huiqin;Wang Hui(Dept of Nuclear Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)
出处
《安徽医科大学学报》
CAS
北大核心
2019年第2期203-206,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省科技厅科技攻关项目(编号:1704a0802164)
