摘要
目的 探讨长期应用酚酞对结肠肌电及肠神经系统 (ENS)的影响。方法 建立大鼠“泻剂结肠”模型 ,应用电生理、组化及免疫组化技术研究酚酞对大鼠结肠动力、ENS多种神经递质及Cajal间质细胞 (ICC)的影响。结果 大鼠饲以酚酞 3个月后 ,结肠慢波频率减慢 ,结肠肌间丛还原型烟酰胺腺嘌呤二核苷磷酸黄递酶阳性神经细胞数目增多 ,乙酰胆碱酯酶阳性神经细胞数目减少 ,一氧化氮合酶免疫反应性增强 ,生长抑素免疫反应性减弱 ,肌间丛ICC分布不均匀 ,突起连接杂乱。结论 长期应用酚酞对结肠动力和ENS有损害作用 ,在临床治疗顽固性便秘时应避免长期应用酚酞等刺激性泻剂。
Objective To explore the effects of phenolphthalein on colonic motility and enteric nervous system. Methods “Cathartic colon” model was established with phenolphthalein, and effects of phenolphthalein on colonic motility, enteric nervous system neurotransmitters, and interstitial cells of Cajal were studied by electrophysiological, histochemical, and immunohistochemical techniques. Results After 3 months of treatment with phenolphthalein in rats, the frequency of slow wave in colon decreased, and the intestinal transit time delayed significantly. In whole mount preparations of myenteric plexus, reduced form of nicotinamide adenine dinucleotide ohosphate positive neurons increased, whereas acetylcholinesterase positive neurons decreased. The immunoreactivity of nitric oxide synthase increased, whereas the immunoreactivity of somatostantin decreased. The distribution of interstitial cells of Cajal (ICC) in myenteric plexus of the cathartic colon was asymmetric, and its processes were intricately connected compared with controls. Conclusion Due to its detrimental effects on colonic motility and enteric nervous system, phenophthalein, a stimulant laxative, should be avoided in the long term treatment of intractable constipation.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2003年第12期723-726,共4页
Chinese Journal of Digestion
