摘要
比较了各种碳水化合物对淡紫拟青霉(Paecilomyces lilacinus)右旋糖酐酶形成的影响,右旋糖酐是最好的碳源,也是最佳诱导物。不同分子量(17.2—1000kD)的右旋糖酐对酶形成的诱导作用不同,酶的产生随右旋糖酐分子量的增大而增加。用分子量为1000kD 的右旋糖酐作碳源时比用17.2kD 的右旋糖酐作碳源时的产酶量高40%以上。用右旋糖酐和其它糖的混合物作碳源时,酶的形成受到不同程度的抑制。右旋糖酐酶形成的其它适宜条件:氮源为牛肉蛋白胨,培养基初始 pH6.0—7.0,种龄为48小时,在250ml 三角瓶中装50ml 培养基,于28℃在200r/min 摇床上培养6天。
Induced formation conditions of dextranase by Paecilomyces lilacinus were investigated.Ef-fect of various carbohydrates on dextranase formation was examined,dextran was the best C-source and as an inducer.The effect of dextran with different molecular weight(from 17.2 to1000 kD)on dextranase formation was compared,productivity of dextranase increased with in-crease of dextran molecular weight.When dextran of 1000kD was used as C-source,The enzymeformation was 40% higher than that 17.2kD dextran.When other sugars were separately add(?)d tothe medium with dextran,the enzyme formation was repressed.Besides C-source,the other optimum conditions of dextranase formation were as folows:N-source,beef peptone;medium initial pH,6.0—7.0;culture temperature,28℃;inoculum amountabout 10%,and the organism was cultivated for 6 days on 200r/min shaker in 250ml flaskerwith 50ml medium.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第5期334-339,共6页
Acta Microbiologica Sinica
