摘要
通过大量实验室工作 ,研究了菌龄、涂片厚薄均匀度、媒染时间、乙醇脱色程度等因素对大肠杆菌革兰氏染色结果的影响。试验结果显示 ,在对大肠杆菌进行革兰氏染色时 ,细菌在伊红美蓝培养基上培养的时间不宜超过 2 4h ;涂片时应为均匀薄片 ;革氏碘液媒染时间以 30s～ 6 0s为宜 ;乙醇脱色程度是革兰氏染色的关键 ,脱色时间应严格控制在 30s～ 6 0s。
In the paper,the influences of bacterium age,the even thickness of smear,mordant agent time,the degree of ethanol decolouration and other related factors on the result of Gram's dye of Coliform are discussed.The result of experiments shows that during Coliform Gram's dye the time of culturing bacterium in EMB medium is within 24h;smear should be even and thin;the time of mordant agent is appropriate between 30s and 60s;the degree of ethanol decolouration is key to experiment and the time of decolouration is strictly controlled between 30s and 60s.
出处
《安徽建筑工业学院学报（自然科学版）》
2003年第4期62-64,共3页
Journal of Anhui Institute of Architecture(Natural Science)
