摘要
探讨粪便脱氧核糖核酸(DNA)中p33生长抑制基因1b(p33ING1b)、染色体连锁凋亡抑制蛋白相关因子1(XAF-1)甲基化对散发性结直肠癌的筛查价值。选取2018年4月至2019年3月该院及广西医科大学第一附属医院结直肠肛门外科收治的散发性结直肠癌60例作为癌症组,同期选取体检良性结直肠息肉60例作为对照组,两组均采用甲基化特异性聚合酶链反应(MSP)检测粪便DNA中p33ING1b、XAF-1甲基化情况。癌症组粪便DNA中p33ING1b、XAF-1甲基化率明显高于对照组,差异有统计学意义(P <0. 05);在筛查散发性结直肠癌敏感度、特异度、准确度中,p33ING1b为73. 33%,80. 00%,76. 67%;XAF-1为60. 00%,73. 33%,66. 67%;p33ING1b联合XAF-1为90. 00%,93. 33%,91. 67%;p33ING1b联合XAF-1明显高于p33ING1b、XAF-1,差异有统计学意义(P <0. 05)。癌症组中,不同性别、年龄、肿瘤直径、分化程度、淋巴结转移的p33ING1b、XAF-1甲基化阳性率比较,差异无统计学意义(P> 0. 05)。不同Dukes分期、浸润深度的p33ING1b、XAF-1甲基化阳性率比较,差异有统计学意义(P <0. 05)。粪便DNA中p33ING1b、XAF-1甲基化与散发性结直肠癌的发生发展有关,可作为筛查散发性结直肠癌的重要指标,且二者联合的价值更高。
To discuss the screening value of methylation of p33 growth suppressor gene 1 b(p33 ING1 b)and chromosome-linked inhibitor of apoptosis protein-related factor 1(XAF-1)in fecal deoxyribonucleic acid(DNA)for sporadic colorectal cancer.60 patients with sporadic colorectal cancer were selected as the cancer group from September 2016 to September 2018 in the author’s hospital;according to the random distribution,60 cases of benign colorectal polyps were selected as control group at the same time.The methylation specific polymerase chain reaction(MSP)was used to detect the methylation of p33 ING1 b and XAF-1 in fecal DNA in the two groups.The methylation rates of p33 ING1 b and XAF-1 in fecal DNA in the cancer group were significantly higher than those in the control group;the difference was significant(P<0.05).In the sensitivity,specificity,accuracy of screening the sporadic colorectal cancer,the p33 ING1 b was 73.33%,80.00%,76.67%respectively;the XAF-1 was 60.00%,73.33%,66.67%respectively;the p33 ING1 b combined with XAF-1 was 90.00%,93.33%,91.67%respectively;the p33 ING1 b combined with XAF-1 was significantly higher than that of the p33 ING1 b and XAF-1;the difference was statistically significant(P<0.05).In cancer group,there was no significant difference in the methylation positive rates of p33 ING1 b and XAF-1 among the different genders,ages,tumor diameters,differentiation degrees,lymph node metastasises(P>0.05),but there was significant difference in the methylation positive rates of p33 ING1 b and XAF-1 among the different Dukes stages and depth of invasion(P<0.05).Methylation of p33 ING1 b and XAF-1 in fecal DNA are related to the occurrence and development of sporadic colorectal cancer,and they can be used as the important indicator for screening sporadic colorectal cancer,and the combination of the two has the higher value.
作者
李振
钟世彪
唐莉
余泽炎
黄体强
闭尔奇
LI Zhen;ZHONG Shi-biao;TANG Li;YU Ze-yan;HUANG Ti-qiang;BI Er-qi(The Affitiated Nationality Hospital of Guangxi Medical Univsesity,Nanning 530001,China)
出处
《药物生物技术》
CAS
2019年第2期119-123,共5页
Pharmaceutical Biotechnology
基金
崇左市科技计划项目(No.崇科FA2018021)
