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Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography 被引量:20

Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography
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摘要 The quality control processes for herbal medicines have been problematic. Flavonoids are the major active components of Huangqin Tang(HQT, a traditional Chinese medicine formula). In this study, we used a combinative method approach consisting of chromatographic fingerprinting(high performance liquid chromatography; HPLC), quantitative methods and a pharmacodynamic evaluation model to analyze the flavonoids of HQT obtained from different sources. Ten batches of HQT were analyzed by the HPLC fingerprinting method and 26 common peaks were detected, of which 23 peaks corresponded with the chemical profile of HQT. In addition, 11 major compounds were identified by LC–MS analysis(liquid chromatography–tandem mass spectrometer; LC–MS^n) and quantified by the HPLC quantitative method approach. The studied10 batches of HQT were found to be homogeneous in their composition with a similarity between 0.990 and1.000. The distribution of the 11 identified compounds was found to be very similar among the batches. Only slight pharmacodynamic differences were detected between the different batches, confirming the homogeneity of HQT. The results of this study prove that the combination of chromatographic fingerprinting and quantitative analysis can be readily used for comprehensive quality control of herbal medicines. The quality control processes for herbal medicines have been problematic. Flavonoids are the major active components of Huangqin Tang (HQT, a traditional Chinese medicine formula). hi this study, we used a combinative method approach consisting of chromatographic fingerprinting (high performance liquid chromatography; HPLC), quantitative methods and a pharmacodynamic evaluation model to analyze the flavonoids of HQT obtained from different sources. Ten batches of HQT were analyzed by the HPLC fingerprinting method and 26 common peaks were detected, of which 23 peaks corresponded with the chemical profile of HQT. In addition, 11 major compounds were identified by LC-MS analysis (liquid chromatography-tandem mass spectrometer; LC-MSn) and quantified by the HPLC quantitative method approach. The studied 10 batches of HQT were found to be homogeneous in their composition with a similarity between 0.990 and 1.000. The distribution of the 11 identified compounds was found to be very similar among the batches. Only slight pharmacodynamic differences were detected between the different batches, confirming the homogeneity of HQT. The results of this study prove that the combination of chromatographic fingerprinting and quantitative analysis can be readily used for comprehensive quality control of herbal medicines. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
出处 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2016年第2期148-157,共10页 药学学报(英文版)
基金 financially supported by the National Natural Science Foundation of China (Nos. 81273662 and 81473592) the Operational Expenses for Basic Research of China Academy of Chinese Medical Sciences (No. ZZ2014020)
关键词 Radix Scutellariae Flavonoids HPLC fingerprinting ANALYSIS Multiple-component quantitative ANALYSIS PAW EDEMA CARRAGEENIN ANTIPYRETIC Anti-inflammatory Radix Scutellariae Flavonoids HPLC fingerprinting analysis Multiple-component quantitative analysis Paw edema Carrageenin Antipyretic Anti-inflammatory
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