摘要
目的建立测定红宝散中葛根素含量的高效液相色谱(HPLC)法。方法以Apollo C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为甲醇-水(25∶75),流速为1.0 mL/min,紫外检测波长为250 nm,柱温为室温。结果葛根素进样量在0.100 1~1.001 0μg范围内与峰面积线性关系良好(r=1.000 0),平均回收率99.76%,RSD=0.30%(n=9)。结论该方法准确、简便、快速,可作为红宝散中葛根素的含量测定方法。
Objective To establish a HPLC method for the determination of puerarin in Hongbao Powders. Methods The chromatograph-ic column was the Apollo C18 column(250 mm × 4. 6 mm,5 μm)and the mobile phase was methanol-water(25 :75). The flow rate was 1. 0 mL/min and the IV detective wavelength was 250 nm. The column temperature was the room temperature. Results The sample size of puerarin in the range of 0. 100 1-1. 001 0 μg showed good linear relation with the peak area( r=1. 000 0),the aver-age recovery rate was 99. 76%,RSD=0. 30%( n=9 ) . Conclusion This method is accurate,simple and rapid,and can be used for the content determination of puerarin in Hongbao Powders.
出处
《中国药业》
CAS
2014年第10期41-42,共2页
China Pharmaceuticals
