摘要
目的研究高碘摄入对仔鼠脑髓鞘化的影响及硒干预的作用。方法将Balb/c小鼠按体重随机分为3组:正常对照组、高碘组(3mg/L)以及高碘+Se组(3mg/L+0.5mg/L Se),分别喂饲高碘水或高碘-硒水以及正常鼠饲料。各组亲代鼠饲养4个月后雌雄按2:1交配,取14d仔鼠血、脑组织测定相关指标。结果与正常对照组相比,高碘仔鼠血清和脑组织TT_4显著下降,TT_3水平亦降低;脑MBP mRNA表达水平较对照组下降了27%。补硒提高仔鼠甲状腺激素的水平,上调仔鼠脑MBP mRNA的表达。结论高碘引起仔鼠甲状腺激素水平降低可能是仔鼠脑MBP mRNA表达水平下降的原因,硒可通过调节甲状腺激素水平从而上调仔鼠脑MBP mRNA的表达。
Objective To study the molecular mechanism of selenium intervention on myelinization in filial mice by RT-PCR. Methods 135 weanling female Balb/C mice were assigned into 3 groups and given drinking water including different doses of iodine and selenium. Normal control (NC, tap water), high iodine intake group (HI, 3.0mg/L I of drinking water), high iodine intake and selenium supplementation group Ⅰ (HI+Se, 3.0mg/L I+0.5mg/L Se of drinking water). All the male and female mice were mated after 4 months later; related indicators filial mice on 14 day were determined. Results In filial mice, compared with NC group, serum TT_4 decreased significantly in HI groups, TT_3 also decreased, and mRNA expression of MBP in cerebrum was down-regulated 27%. The selenium supplementation groups inhibited the decrease of TT_4 and TT_3, up regulated the mRNA expression of MBP in cerebrum. Conclusion High-Iodine Intake can decrease thyroxine of filial mice, which maybe is the reason for down-regulated mRNA expression of MBP in cerebrum, and selenium can be some intervention by thyroxine, but need to verify more.
出处
《营养健康新观察》
2004年第4期12-16,共5页
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