摘要
目的 探讨低频经颅磁刺激对颞叶癫痫大鼠病灶区颞叶和海马的细胞凋亡有无抑制作用。 方法 制作氯化锂 匹罗卡品所致的慢性期颞叶癫痫大鼠动物模型 ,分为两组 ,一组给予磁场强度 0 .4T ,刺激时程 0 .2ms的低频 (0 .5Hz)经颅磁刺激 ,另一组给予“假性”刺激。以原位末端标记法 (TUNEL)标记DNA片段 ,电子显微镜检测两组颞叶和海马的细胞凋亡情况 ,并分别与对照组进行比较。结果 在慢性期颞叶癫痫大鼠的颞叶和海马均可见大量凋亡细胞。在给予适量的低频经颅磁刺激后 ,相应脑部的凋亡细胞数均明显减少 (P <0 .0 5 )。 结论 适量的低频经颅磁刺激能抑制颞叶癫痫大鼠的细胞凋亡 ,对癫痫所致的脑部损伤有修复作用 ,是一种有前途的治疗癫痫的新疗法。
Objective To explore whether low-frequency transcranial magnetic stimulation could inhibit the neuronal apoptosis in temporal lobe and hippocampus of chronic temporal lobe epilepsy rats. Methods With the chronic temporal lobe epilepsy rat model induced by lithium-pilocarpine, temporal lobe and hippocampus neuronal apoptosis were detected by in situ TUNEL assay and electric microscopy in two temporal lobe epilepsy groups, one of which was given transcranial magnetic stimulation. Results Compared with normal control group, the apoptosis increased obviously in temporal lobe and hippocampus of temporal epilepsy rats. After transcranial magnetic stimulation was administrated, neuronal apoptosis decreased significantly in temporal lobe and hippocampus in temporal lobe epilepsy groups. Conclusion Appropriate transcranial magnetic stimulation inhibits neuronal apoptosis in temporal lobe and hippocampus of temporal lobe epilepsy rat and has a neuroprotective effect against brain damage induced by epilepsy.
基金
天津市自然科学基金项目 (0 2 3 613 911)
