摘要
在显微操作器上微分离杉木(Cunninghamialanceolata(Lamb.)Hook)的4条单染色体,以简并寡核苷酸为引物进行PCR扩增(DOP PCR),并将其DOP PCR产物进行RAPD分析。结果表明,不同染色体扩增产物间有明显多态性;用RAPD PCR的方式可反应出不同染色体遗传信息的差异,从这些差异中可能找到属于某单染色体的特异信息,进而在分子水平上对染色体进行编号。
Four single chromosome of Cunninghamia lanceolata was dissected under the micromanipulator.After degenerate oligonucleotideprimedPCR amplification(DOPPCR),DNA product were tested with RAPD.The results indicated: the polymorphism among different single microdissected Cunninghamia lanceolata chromosome was evident.This research provides a new method to identify microdissected Cunninghamia lanceolata chromosome and locate gene to chromosome of Cunninghamia lanceolata chromsome.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第3期55-58,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(39970626)
