摘要
[目的]探讨反义核酸对周期素D1的表达及对胃癌细胞增殖的影响。[方法]应用免疫荧光组织化学方法结合激光共聚焦显微镜,观察了人胃腺癌BGC鄄823细胞株反义核酸处理前后周期素D1的表达。[结果]反义核酸处理组周期素D1的表达较空白对照组明显降低。[结论]反义核酸抑制周期素D1的表达并抑制胃癌细胞的增殖。
To investigate the impact of antisense oligodeoxynucleotide of cyclin D 1 (ASODN)expression on cell proliferation in human stomach adenocarcinoma cell BGC-823cell line.The exepression of cyclin D 1 in hu-man stomach adenocarcinoma cell BGC-823cell line was measured by immunofluorescent histochemistry.The ex-pression of ASODN was significantly lower than that of control.[Conclusion]ASODN could specific inhibit the expression of cyclin D 1 protein and regulate cell cycle and cell proliferation in BGC-823cells.
出处
《中国肿瘤》
CAS
2003年第5期296-297,共2页
China Cancer
关键词
胃肿瘤
反义核酸
细胞周期素
激光共聚焦显微镜
免疫组化
stomach neoplasms
antisense oligodexynucleotide
cyclin
confocal microscope
immunofluorscence
