摘要
In vitro regeneration systems of Atrichum mosses, Atrichurn undulatum (Hedw.) P. Beauv. and A. undulatum var. minus (Hedw.) Par. were established. After one month, soft, friable and green calli were induced successfully from inoculated protonema of Atrichum mosses on MS medium containing glucose (4%) and 6-BA (0.2-2.0 mg/L). The suitable culture medium for the callus induction and regular subculture was MS medium with 1.0-2.0 mg/L 6-BA and 4% glucose. The calli of Atrichum mosses developed into protonema, when it was transferred to phytohormone-free MS medium with 4% glucose. Meanwhile, the calli developed into erect gametophytes through protonema phase on carbohydrate-free Benecke medium.
报道了仙鹤藓(Atrichum undulatum(Hedw.)P.Beauv.)和仙鹤藓小形变种(Atrichum undulatum var.minus(Hedw.)Par.)的组织培养再生体系的建立.为研究仙鹤藓属(Atrichum)藓类愈伤组织的诱导和再分化,将仙鹤鲜和仙鹤藓小形变种原丝体接种在含有4%葡萄糖和0.2～2.0 mg/L 6-BA的MS培养基上,培养一个月后,成功地诱导出疏松、易碎的绿色愈伤组织.愈伤组织诱导和常规继代培养较适合的培养基为含4%葡萄糖和1～2 mg/L6-BA的MS培养基.当将继代培养5次的脱分化藓类愈伤组织转移到含4%葡萄糖但无任何激素的MS培养基上时,能再分化形成原丝体,而在无任何碳源的Benecke培养基上培养时,能再分化形成经原丝体阶段发育来的直立配子体.
基金
北京市自然科学基金(5982007)。~~
