摘要
目的探讨猫棒束孢Isariafelina(IF)对环磷酰胺所致免疫低下小鼠免疫功能的影响。方法将昆明种小鼠随机分为6组,分别为对照组、模型组、阳性对照组(香菇多糖,200 mg/kg)及IF高、中、低剂量(400、200、100 mg/kg)组。除对照组外,其余5组小鼠ip环磷酰胺制备免疫低下小鼠模型,每日ig给药1次,共给药10 d。给药结束后,检测小鼠体质量增长值、肝脏指数、脾脏指数、胸腺指数、血常规指标;碳廓清法和迟发型变态反应(DTH)测定小鼠非特异性免疫功能和细胞免疫功能;双抗体夹心ELISA法测定小鼠血清中肿瘤坏死因子-α(TNF-α)水平。结果与对照组比较,模型组小鼠体质量增长值,脾脏指数,白细胞、红细胞等血常规指标,吞噬指数(α),足跖厚度差,TNF-α水平显著降低(P<0.05),表明环磷酰胺造免疫低下小鼠模型制备成功。与模型组比较,IF各剂量组小鼠的脾脏指数,白细胞、红细胞等血常规指标,α,足跖厚度差,TNF-α水平均显著升高(P<0.05)。结论 IF对环磷酰胺所致免疫低下模型小鼠具有免疫保护作用。
Objective To investigate the effects of Isaria felina(IF)on immune function in mice with immunosuppression induced by cyclophosphamide.Methods The kunming mice were randomly divided into control group,model group,positive group(lentinan,200 mg/kg),high,medium,and low dose groups of IF(400,200,100 mg/kg).Except for the control group,the other five groups were intraperitoneally injected with cyclophosphamide to establish an immunosuppression mouse model.The drug was administered once a day for a total of 10 d.Then the body mass growth,liver index,thymus index,spleen index and blood routine indexes were examined;The carbon profile method and the delayed immune response(DTH)was used to determine the non-specific immune function and the cellular immune function in mice;The content of TNF-αin serum of mice was determined by double antibody sandwich ELISA.Results Compared with the control group,the growth value of body mass,spleen index,white blood cells,red blood ce lls and other blood routine indexes,phagocytic index,plantar thickness difference and TNF-αcontent of model group were significantly reduced(P<0.05),which suggested that the establishmeng of immunocompromised mouse models induced by cyclophosphamide was successful.Compared with the model group,spleen index,white blood cells,red blood cells and other blood routine indexes,phagocytic index,plantar thickness difference and TNF-αcontent in each dose group of IF were significantly increased(P<0.05).Conclusion I.felina has immunoprotective effects on mice with immunodeficiency induced by cyclophosphamide.
作者
王跃凤
杨喜花
陈丽霞
阎磊
王靖
张生万
WANG Yue-feng;YANG Xi-hua;CHEN Li-xia;YAN Lei;WANG Jing;ZHANG Sheng-wan(College of Life Sciences,Shanxi University,Taiyuan 030006,China;Department of Comparative Medicine,Shanxi Cancer Institute,Taiyuan 030013,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第11期2651-2655,共5页
Chinese Traditional and Herbal Drugs
基金
动物实验室增项建设(2015006)
