摘要
苎麻又叫"中国草",是我国特有的纤维兼饲用的经济作物。根腐线虫是苎麻的主要病害之一,对纤维产量造成了严重的损失。本研究以中苎一号为材料,采用盆栽模拟试验,研究了根腐线虫胁迫后苎麻产量性状及生理指标的变化。结果表明,根腐线虫胁迫对苎麻产量影响很大,接种时间越长危害越大;根腐线虫胁迫的时间越长多酚氧化酶(PPO)活性越高,但一定的时间之后增幅差异不显著,过氧化物酶(POD)的变化趋势与多酚氧化酶的变化相似;过氧化氢酶(CAT)活性在不同胁迫时间呈现显著上升的趋势,而超氧化物歧化酶(SOD)活性的变化呈现先上升后有所下降的变化趋势;苎麻在遭受根腐线虫胁迫后,在次生代谢物最明显的变化是单宁和总酚类化合物含量的增加。本研究明确了苎麻根腐线虫胁迫下产量及生理活性等变化特点,为苎麻根腐线虫的防治及抗性指标的筛选提供理论基础。
Ramie( Boehmeria nivea),commonly known as China grass,is one of the most important natural fiber and forage economic crops. The root-lesion nematode is a major ramie disease and causes large fiber yield losses in China annually. In order to investigate ramie yield and physiological responses to root-lesion nematode stress,three groups of potted ramie( RLN-infected for 30 days( Inf1),severe RLN-infected stress( RLN-infected for 60days) and normal control) were tested. The result showed that the root-lesion nematode infection in ramie could lead to great yield loss. An increase of the polyphenol oxidase( PPO) activity was commonly observed in root-lesion nematode stressed,but the differences were indistinctively among them. The change trend of peroxidase( POD) was similar to the changes of polyphenol oxidase( PPO). Catalase( CAT) activity in different stress time presented the significant trend of increasing,and changes of superoxide dismutase( SOD) activity after rising to the change trend of decline. The most significant change was that there was an increase in tannin and total phenols content on secondary metabolites after RLN-infected stress. In this study,it was clear that the yield and physiological activity changes of ramie in response to root-lesion nematode infection,and it provided theoretical basis to prevent the root-lesion nematode and screening of resistance index.
出处
《中国麻业科学》
2016年第4期162-166,共5页
Plant Fiber Sciences in China
基金
国家自然科学基金(3157102042)
现代农业产业技术体系(CARS-19-E13)
关键词
苎麻
根腐线虫
生理指标
代谢产物
ramie
root-lesion nematode
physiological indexes
metabolites
