摘要
以呼和浩特市某牛场地表土壤为研究对象,通过传统的微生物培养方法和基于16S r RNA基因序列的新型分子生物学方法对其中的微生物进行了分析。结果显示在牛场地表土壤中共鉴定出蜡样芽孢杆菌(Bacillus cereus)、苏云金芽孢杆菌(Bacillus thuringiensis)、地衣芽孢杆菌(Bacillus lincheniformis)、产芽孢梭菌(Clostridium sporogenes)、纯黄肠球菌(Enterococcus gilvus)和拜氏梭菌(Clostridium beijerinckii)共40株,并通过16S r RNA基因序列的系统发育树研究得到蜡样芽孢杆菌、苏云金芽孢杆菌、地衣芽孢杆菌这三种优势芽孢杆菌之间的亲缘关系。该研究为后续开发应用于无抗饲料添加剂和生物农药的绿色、安全、高效的益生芽孢杆菌微生态制剂提供一定的数据基础。
This study chose the surface soil of a dairy farm as the research object, and analyzed the bacterium of the surface soil by traditional culture methods and molecular biology technique based on 16S rRNA genetic sequence. The results in present study showed that there were 40 strains of bacterium in sample including Bacillus cereus, Bacillusthuringiensis, Bacilluslincheniformis, Clostridiumsporogenes, Enterococcusgilvus and Clostridium beijerinckii. Furthermore, the first three was predominant Bacillus in the surface soil of dairy farm and the phylogenetic tree diagram of the genome sequence reveal the genetic relationship among these predominant Bacillus. Thecurrentstudyaimedtoprovidecertainreferenceforexploringhealthy,safetyandefifcientlyprobioticBacillus appliedtoAntibiotics-freefeedingadditivesandBiopesticide.
出处
《中国奶牛》
2014年第Z3期43-48,共6页
China Dairy Cattle
基金
农业部现代农业产业技术体系建设项目(CARS-37)
